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Citation
Engels, W.R., Johnson-Schlitz, D.M., Eggleston, W.B., Sved, J. (1990). High-frequency P element loss in Drosophila is homolog dependent.  Cell 62(): 515--525.
FlyBase ID
FBrf0051376
Publication Type
Research paper
Abstract

P transposable elements in Drosophila melanogaster can undergo precise loss at a rate exceeding 13% per generation. The process is similar to gene conversion in its requirement for a homolog that is wild type at the insertion site and in its reduced frequency when pairing between the homologs is inhibited. However, it differs from classical gene conversion by its high frequency, its requirement for P transposase, its unidirectionality, and its occurrence in somatic and premeiotic cells. Our results suggest a model of P element transposition in which jumps occur by a "cut-and-paste" mechanism but are followed by double-strand gap repair to restore the P element at the donor site. The results also suggest a technique for site-directed mutagenesis in Drosophila.

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell
    Title
    Cell
    Publication Year
    1974-
    ISBN/ISSN
    0092-8674
    Data From Reference
    Aberrations (1)
    Alleles (7)
    Genes (4)
    Natural transposons (2)
    Insertions (5)
    Transgenic Constructs (3)