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Reference
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| Citation |
Kania, M.A., Bonner, A.S., Duffy, J.B., Gergen, J.P. (1990). The Drosophila segmentation gene runt encodes a novel nuclear regulatory protein that is also expressed in the developing
nervous system. Genes Dev. 4(): 1701--1713. |
| FlyBase ID |
FBrf0051834 |
| Type of publication |
Research paper |
| Offprint Available |
Yes |
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External Crossreferences
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| PubMed ID |
2249771 |
| PubMed Abstract |
Generation of the anterior-posterior body pattern in the Drosophila embryo requires the activity of the segmentation genes.
The segmentation gene runt has been classified as one of the primary pair-rule genes because of the pivotal role it plays
in regulating the expression of other pair-rule genes. Here, we present the structure of this gene and describe the pattern
of runt protein expression during embryogenesis. The deduced protein sequence shows no obvious overall homology with any sequences
in the data base. The absence of an identifiable transcription factor motif (e.g., homeo box, zinc finger, leucine zipper,
or helix-loop-helix) makes runt different from the other early-acting segmentation proteins. A runt-specific polyclonal antibody
was generated and used to demonstrate that the subcellular location of the protein is in the nucleus. Double-staining immunolocalization
experiments were used to determine the overlap of the runt protein pattern with the patterns of the pair-rule genes hairy
(h), even-skipped (eve), and fushi tarazu (ftz). We found that the patterns of runt and hairy are complementary. Their phasing
is shifted anteriorly by two cell diameters with respect to the complementary eve and ftz patterns. Experiments with the runt
antibody also indicated that the protein is present throughout embryogenesis and is expressed extensively in the developing
central and peripheral nervous system.
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Associated Information
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| Text of personal communication |
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Related Publications
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Also Published As
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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Published In
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| Abbreviation |
Genes Dev. |
| Title |
Genes & Development |
| Authors |
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| Volume range |
1- |
| Year range |
1987- |
| Page range |
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| Publisher |
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| Place of publication |
Cold Spring Harbor, NY |
| Language of publication |
English |
| ISBN/ISSN |
0890-9369 |
| CODEN |
GEDEEP |
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Data from Reference
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| Aberrations (1)
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| Alleles (3)
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| Balancers (2)
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| Genes (6)
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| Insertions (1)
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