Reference Report

Reference
Citation	Thisse, C., Perrin-Schmitt, F., Stoetzel, C., Thisse, B. (1991). Sequence-specific transactivation of the Drosophila twist gene by the dorsal gene product. Cell 65(): 1191--1201. (Export to RIS)
FlyBase ID	FBrf0053349
Publication Type	Research paper
PubMed ID	1648449
PubMed Abstract	The maternal gene dorsal encodes a nuclear protein acting as a morphogen that determines the size and fate of regions along the dorsal-ventral axis of the Drosophila embryo. From previous genetic and biochemical studies it was hypothesized that dorsal might be responsible for the activation of the zygotic gene twist. In this report, regulatory sequences required for correct spatial and quantitative expression of twist are defined, by using phenotypic rescue and studying twist-beta-galactosidase expression. In addition, by transient cotransfection assays, we show that the dorsal protein specifically activates expression from the twist promoter. We demonstrate that dorsal is a sequence-specific DNA-binding protein that recognizes a motif similar to that recognized by the mammalian transcriptional activator NF-kappa B.
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	Cell
Title	Cell
Publication Year	1974-
ISBN/ISSN	0092-8674
Data from Reference
Alleles (56)
	dl^Act5C.PT Ecol\CAT^a.twi.Bg-C Ecol\CAT^a.twi.Bs-H Ecol\CAT^a.twi.Bs-P Ecol\CAT^a.twi.C-B Ecol\CAT^a.twi.C-E Ecol\CAT^a.twi.E-B Ecol\CAT^a.twi.P-Bg Ecol\CAT^a.twi.P-Bs Ecol\CAT^a.twi.P-H Ecol\CAT^twi.88P Ecol\CAT^twi.251P Ecol\CAT^twi.440P Ecol\CAT^twi.610P Ecol\CAT^twi.770P Ecol\CAT^twi.1220P Ecol\CAT^twi.1450P Ecol\CAT^twi.1750P Ecol\CAT^twi.2450P Ecol\CAT^twi.2850P Ecol\CAT^twi.3000P Ecol\CAT^twi.Bg-C Ecol\CAT^twi.Bs-H Ecol\CAT^twi.Bs-P Ecol\CAT^twi.C-B Ecol\CAT^twi.C-E Ecol\CAT^twi.E-B Ecol\CAT^twi.P-Bg Ecol\CAT^twi.P-Bs Ecol\CAT^twi.P-H Ecol\CAT^twi.X-B Ecol\CAT^twi.X-P Ecol\lacZ^twi.0.8a Ecol\lacZ^twi.0.8t Ecol\lacZ^twi.1.4a Ecol\lacZ^twi.1.4t Ecol\lacZ^twi.3a Ecol\lacZ^twi.3t Ecol\lacZ^twi.5a Ecol\lacZ^twi.5t Tl⁸ twi^+t9.2 twi^ey53 twi^ey62 twi^RY50 twi^T10 twi^T11 twi^T36 twi^T40 twi^T48 twi^T64 twi^T71 twi^T72 twi^T135 twi^v50 w^+mW.hs
Constructs (48)
	001196 001204 001205 blcat.mcs p3CAT P{twi-βgal-0.8a} P{twi-βgal-0.8t} P{twi-βgal-1.4a} P{twi-βgal-1.4t} P{twi-βgal-3.0a} P{twi-βgal-3.0t} P{twi-βgal-5.0a} P{twi-βgal-5.0t} P{WT9} P{WT10} P{WT11} P{WT36} P{WT40} P{WT48} P{WT64} P{WT71} P{WT72} P{WT135} pACH pBLCAT2 pBLCAT3 pP{twi-βgal-0.8a} pP{twi-βgal-0.8t} pP{twi-βgal-1.4a} pP{twi-βgal-1.4t} pP{twi-βgal-3.0a} pP{twi-βgal-3.0t} pP{twi-βgal-5.0a} pP{twi-βgal-5.0t} pP{WT9} pP{WT10} pP{WT11} pP{WT36} pP{WT40} pP{WT48} pP{WT64} pP{WT71} pP{WT72} pP{WT135} pPAC-dorsal pSCAT0 ptwi(a)-CAT ptwi-CAT
Genes (7)
	dl Ecol\CAT Ecol\lacZ Tl tsg twi w
Sequence features (7)
	twi-protein_bind-1 twi-protein_bind-4 twi-protein_bind-5 twi-protein_bind-6 twi-protein_bind-13 twi-protein_bind-15 twi-protein_bind-16
Transcripts (2)
	Ecol\lacZ^twi.0.8tRA Ecol\lacZ^twi.1.4tRA