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Reference Report
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| Citation | Dynlacht, B.D., Hoey, T., Tjian, R. (1991). Isolation of coactivators associated with the TATA-binding protein that mediate transcriptional activation. Cell 66(): 563--576. (Export to RIS) | ||
| FlyBase ID | FBrf0053382 | ||
| Publication Type | Research paper | ||
| PubMed ID | 1907890 | ||
| PubMed Abstract | A key step in the regulation of transcription involves interactions between promoter-selective factors and various components of the transcriptional apparatus. Here we report the requirements for transcriptional activation directed by NTF-1, a developmentally regulated transcription factor in Drosophila. Reconstituted transcription with fractionated Drosophila basal factors reveals that activation by NTF-1 requires factors present in the endogenous TFIID fraction that are distinct from the purified TATA-binding protein (TBP). Glycerol gradient sedimentation and immunoprecipitation analyses indicate that TFIID is a multiprotein complex containing TBP and at least six tightly bound TBP-associated factors (TAFs). Preparations of TBP lacking TAFs after fractionation with denaturants no longer support activation by NTF-1 but retain basal level activity. Addition of immunopurified and renatured TAFs to free TBP restores the ability of NTF-1 to activate transcription without influencing basal transcription. These results suggest that one or more of the TAF polypeptides confer coactivator function. | ||
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| Language of Publication | English | ||
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| Publication Type | Journal | ||
| Abbreviation | Cell | ||
| Title | Cell | ||
| Publication Year | 1974- | ||
| ISBN/ISSN | 0092-8674 | ||
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Data from Reference
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| Genes (7)
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