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Smoller, D.A., Petrov, D., Hartl, D.L. (1991). Characterization of bacteriophage P1 library containing inserts of Drosophila DNA of 75-100 kilobase pairs.  Chromosoma 100(): 487--494.
FlyBase ID
FBrf0053438
Publication Type
Research paper
Abstract

A multiple-hit bacteriophage P1 library containing DNA fragments from Drosophila melanogaster in the size range 75-100 kb was created and subjected to a preliminary evaluation for completeness, randomness, fidelity, and clone stability. This P1 library presently contains 3840 individual clones, or approximately two genome equivalents. The library was screened with a small set of unique-sequence test probes, and clones containing the sequences have been recovered. In situ hybridization with salivary gland chromosomes indicates that the clones originate from the site of the probe sequences in the genome, and filter hybridization of restriction digests suggests that the clones are not rearranged in comparison with the genomic sequences. Approximately 1.7% of the clones contain sequences that hybridize with ribosomal DNA. A small subset of these clones was tested for stability by examination of restriction fragments produced after repeated subculturing, and no evidence for instability was found. The P1 cloning system has general utility in molecular genetics and may provide an important intermediate level of resolution in physical mapping of the Drosophila genome.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Chromosoma
    Title
    Chromosoma
    Publication Year
    1939-
    ISBN/ISSN
    0009-5915
    Data From Reference
    Genes (4)
    Datasets (3)