Reference Report

Reference
Citation	Morrissey, D., Askew, D., Raj, L., Weir, M. (1991). Functional dissection of the paired segmentation gene in Drosophila embryos. Genes Dev. 5(): 1684--1696. (Export to RIS)
FlyBase ID	FBrf0054038
Publication Type	Research paper
PubMed ID	1679407
PubMed Abstract	An ectopic expression assay in Drosophila embryos was used to investigate the roles of pair-rule segmentation genes in the spatial regulation of the segment-polarity gene, engrailed (en). It is hypothesized that the regions of overlap in expression of two genes, paired (prd) and even-skipped (eve), define the odd-numbered en expression stripes. Consistent with this combinatorial model, ectopic expression of prd caused these en stripes to be expanded posteriorly. Surprisingly, however, ectopic expression of a prd gene with a deletion of the conserved paired box resulted in loss of these odd-numbered en stripes. This dominant negative effect is a phenocopy of en expression in prd embryos and suggests that the paired box is necessary for normal prd- function. A similar deletion of odd-numbered en stripes was also observed after ectopic expression of a chimeric fushi tarazu (ftz) gene containing a substituted prd gene homeo box; in addition, in these embryos, the even-numbered en stripes were expanded anteriorly, as observed when the unaltered ftz gene is ectopically expressed. These effects suggest that the chimeric protein may have DNA or protein targets of both the normal Ftz and Prd proteins.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Aberrations (2)
	Df(2R)eve In(2LR)O
Alleles (5)
	ftz::prd^hs.PM prd^hs.PM prd^ΔPB.hs ry^+t7.2 w^+mW.hs
Balancers (1)
	CyO-Thb8
Constructs (3)
	P{hs-ftz/prdHD/ftz} P{hs-prd.M} P{hs-prdΔPB}
Genes (8)
	en eve ftz ftz::prd gsb prd ry w
Insertions (1)
	P{Thb8-lacZ}DM1