Reference Report

Reference
Citation	Rasooly, R.S., New, C.M., Zhang, P., Hawley, R.S., Baker, B.S. (1991). The lethal(1)TW6^cs mutation of Drosophila melanogaster is a dominant antimorphic allele of nod and is associated with a single base change in the putative ATP-binding domain. Genetics 129(): 409--422. (Export to RIS)
FlyBase ID	FBrf0054154
Publication Type	Research paper
PubMed ID	1743485
PubMed Abstract	The l(1)TW-6cs mutation is a cold-sensitive recessive lethal mutation in Drosophila melanogaster, that affects both meiotic and mitotic chromosome segregation. We report the isolation of three revertants of this mutation. All three revert both the meiotic and mitotic effects as well as the cold sensitivity, demonstrating that all three phenotypes are due to a single lesion. We further show that these revertants fail to complement an amorphic allele of the nod (no distributive disjunction) locus, which encodes a kinesin-like protein. These experiments demonstrate that l(1)TW-6cs is an antimorphic allele of nod, and we rename it nodDTW. Sequencing of the nod locus on a nodDTW-bearing chromosome reveals a single base change in the putative ATP-binding region of the motor domain of nod. Recessive, loss-of-function mutations at the nod locus specifically disrupt the segregation of nonexchange chromosomes in female meiosis. We demonstrate that, at 23.5 degrees, the meiotic defects in nodDTW/+ females are similar to those observed in nod/nod females; that is, the segregation of nonexchange chromosomes is abnormal. However, in nodDTW/nodDTW females, or in nodDTW/+ females at 18 degrees, we observe a more severe meiotic defect that apparently affects the segregation of both exchange and nonexchange chromosomes. In addition, nodDTW homozygotes and hemizygous males have previously been shown to exhibit mitotic defects including somatic chromosome breakage and loss. We propose that the defective protein encoded by the nodDTW allele interferes with proper chromosome movement during both meiosis and mitosis, perhaps by binding irreversibly to microtubules.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (9)
	Df(1)N71 Df(1)nod Df(1)v65b Dp(1;2)v⁺65b Dp(1;3)v⁺74c Dp(1;Y)y⁺v⁺#3 In(1)FM7 Tp(1;2)v⁺65b Tp(1;3)v⁺74c
Alleles (6)
	nod⁴ nod^a nod^DR1 nod^DR2 nod^DR3 nod^DTW
Balancers (2)
	FM7a FM7a-nod⁴
Genes (3)
	m nod v