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Citation
ffrench-Constant, R.H., Mortlock, D.P., Shaffer, C.D., MacIntyre, R.J., Roush, R.T. (1991). Molecular cloning and transformation of cyclodiene resistance in Drosophila: an invertebrate gamma-aminobutyric acid subtype A receptor locus.  Proc. Natl. Acad. Sci. U.S.A. 88(): 7209--7213.
FlyBase ID
FBrf0055003
Publication Type
Research paper
Abstract

Cyclodiene resistance represents 60% of the reported cases of insecticide resistance and is also present in vertebrates. Resistance is due to insensitivity of the cyclodiene/picrotoxinin binding site on the gamma-aminobutyric acid subtype A (GABAA) receptor-chloride ionophore complex. Following isolation of cyclodiene-resistant Drosophila mutants, we report the cloning of the locus conferring resistance via a "chromosomal walk" and rescue of the susceptible phenotype by P-element-mediated germ-line transformation. Amino acid sequence analysis of a cDNA from the locus reveals homology with vertebrate GABAA subunits. To our knowledge, this represents the first cloning of an invertebrate GABA receptor and also allows us to manipulate the resistance status of an insect via germ-line transformation. This gene may be useful as a selectable marker in other insect systems.

PubMed ID
PubMed Central ID
PMC52263 (PMC) (EuropePMC)
Related Publication(s)
Erratum

Molecular cloning and transformation of cyclodiene resistance in Drosophila: an invertebrate gamma-aminobutyric acid subtype A receptor locus.
ffrench-Constant et al., 1992, Proc. Natl. Acad. Sci. U.S.A. 89(16): 7849 [FBrf0067296]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Proc. Natl. Acad. Sci. U.S.A.
    Title
    Proceedings of the National Academy of Sciences of the United States of America
    Publication Year
    1915-
    ISBN/ISSN
    0027-8424
    Data From Reference
    Aberrations (4)
    Alleles (5)
    Genes (1)
    Transgenic Constructs (1)