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Citation
Silva, F.J., Bel, Y., Botella, L.M., Cotton, R.G., Ferre, J. (1992). Immunological detection of phenylalanine hydroxylase protein in Drosophila melanogaster.  Biochem. J. 287(1): 85--89.
FlyBase ID
FBrf0055435
Publication Type
Research paper
Abstract

A monoclonal antibody raised against monkey liver phenylalanine hydroxylase (PAH) has been used to detect this protein in Drosophila melanogaster. A cross-reacting material (CRM) band of apparent molecular mass 50-52 kDa, equivalent to that deduced for the Drosophila melanogaster PAH protein based on the pah gene cDNA sequence, has been detected. This CRM was analysed throughout development and showed an equivalent pattern to that reported for PAH activity in this insect, with maxima at pupariation and at pharate adult formation. Distribution of this CRM in larval tissues, the haemolymph and the adult body is mainly restricted to the larval fat body and the adult head. Demonstration of this CRM as the PAH protein comes from the correlation between the decreased PAH enzyme activities of two mutant strains and their decreased amounts of CRM by Western blotting.

PubMed ID
PubMed Central ID
PMC1133127 (PMC) (EuropePMC)
DOI
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Biochem. J.
    Title
    The Biochemical Journal
    Publication Year
    1906-
    ISBN/ISSN
    0264-6021
    Data From Reference
    Alleles (2)
    Genes (2)