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Citation	Kassis, J.A., Noll, E., VanSickle, E.P., Odenwald, W.F., Perrimon, N. (1992). Altering the insertional specificity of a Drosophila transposable element. Proc. Natl. Acad. Sci. USA 89(): 1919--1923.
FlyBase ID	FBrf0057521
Type of publication	Research paper
Offprint Available	Yes
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PubMed ID	1311855
PubMed Abstract	Vectors derived from the Drosophila P element transposon are widely used to make transgenic Drosophila. Insertion of most P-element-derived vectors is nonrandom, but they exhibit a broad specificity of target sites. During experiments to identify cis-acting regulatory elements of the Drosophila segmentation gene engrailed, we identified a fragment of engrailed DNA that, when included within a P-element vector, strikingly alters the specificity of target sites. P-element vectors that contain this fragment of engrailed regulatory DNA insert at a high frequency near genes expressed in stripes.
Biosis	93133800
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Language of publication	English
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Abbreviation	Proc. Natl. Acad. Sci. USA
Title	Proceedings of the National Academy of Sciences of the United States of America
Authors
Volume range	1-
Year range	1915-
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Publisher
Place of publication	Washington, DC
Language of publication	English
ISBN/ISSN	0027-8424
CODEN	PNASA6
Data from Reference
Aberrations (1)
	In(2LR)O
Alleles (21)
	Ecol\lacZ^Dvir\en.J Ecol\lacZ^en-14 Ecol\lacZ^en.96 Ecol\lacZ^en.A Ecol\lacZ^en.B Ecol\lacZ^en.C Ecol\lacZ^en.D Ecol\lacZ^en.E Ecol\lacZ^en.F Ecol\lacZ^en.G Ecol\lacZ^en.H Ecol\lacZ^en.HoxA Ecol\lacZ^en.I Ecol\lacZ^h-C5 Ecol\lacZ^hs.PKa Ecol\lacZ^opa.e Ecol\lacZ^wg-en11 h^C5 ry^+t7.2 w^+mC wg^en11
Balancers (1)
	CyO-P{en1}wg^en11
Constructs (22)
	P{en.96-HZ73(ry)} P{en.96-HZ73(w)} P{en1} P{en2} P{en3} P{enB} P{enC} P{enD} P{enE} P{enF} P{enG} P{enH(w)} P{enH+Hox.A} P{enH+Hox.B} P{enH} P{enI} P{enJ} P{hsp-lacZ} P{hsp} P{opa-en} pP{hsp-lacZ} pP{opa-en}
Genes (6)
	Ecol\lacZ en h ry w wg
Insertions (3)
	P{en1}48A P{en1}wg^en11 P{enC}h^C5