Reference Report

Reference
Citation	Tietze, K., Oellers, N., Knust, E. (1992). Enhancer of split^D, a dominant mutation of Drosophila, and its use in the study of functional domains of a helix-loop-helix protein. Proc. Natl. Acad. Sci. U.S.A. 89(): 6152--6156. (Export to RIS)
FlyBase ID	FBrf0057539
Publication Type	Research paper
PubMed ID	1631102
PubMed Abstract	Helix-loop-helix proteins play important roles in developmental processes, such as myogenesis, neurogenesis, and sex determination. The gene Enhancer of split [E(spl)] of Drosophila, a member of a gene complex that is involved in early neurogenesis, encodes a protein with a basic domain and a helix-loop-helix motif. We took advantage of a dominant mutation of this gene, E(spl)D, to define in vivo structural features of this protein for proper function. The mutation renders the otherwise recessive eye phenotype of spl dominant. By germ-line transformation of different in vitro mutagenized versions of the E(spl) gene, we could demonstrate that the basic domain of this helix-loop-helix protein is functional and necessary for expression of the dominant phenotype. These results are supported by in vitro DNA-binding assays, which showed that the basic domain is in fact necessary for DNA binding, despite the presence of a proline residue. Furthermore, we could show that the dominant enhancement of spl is caused by truncation of the E(SPL)D protein in combination with deletion of a putative regulatory element.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Aberrations (1)
	Df(3R)gro^ry78R-r171.1
Alleles (17)
	E(spl)m8-HLH^1,bd- E(spl)m8-HLH^1,P-N E(spl)m8-HLH^1,P-T E(spl)m8-HLH^1,stop E(spl)m8-HLH^1:+ E(spl)m8-HLH^1.tKa E(spl)m8-HLH¹ E(spl)m8-HLH^+,bd- E(spl)m8-HLH^+,del E(spl)m8-HLH^+,P-N E(spl)m8-HLH^+,P-T E(spl)m8-HLH^+,stop E(spl)m8-HLH^+:1 E(spl)m8-HLH^+t3.1 gro^B48 gro^D w^+mW.hs
Constructs (14)
	P{E(spl)^1,bd-} P{E(spl)^1,P-N} P{E(spl)^1,P-T} P{E(spl)^1,stop} P{E(spl)^1/+} P{E(spl)¹} P{E(spl)^+,bd-} P{E(spl)^+,del} P{E(spl)^+,P-N} P{E(spl)^+,P-T} P{E(spl)^+,stop} P{E(spl)^+/1} P{E(spl)⁺3.1} P{gro^D}
Genes (3)
	E(spl)m8-HLH gro w