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Reference
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| Citation |
England, B.P., Admon, A., Tjian, R. (1992). Cloning of Drosophila transcription factor Adf-1 reveals homology to Myb oncoproteins. Proc. Natl. Acad. Sci. USA 89(): 683--687. |
| FlyBase ID |
FBrf0057548 |
| Type of publication |
Research paper |
| Offprint Available |
Yes |
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External Crossreferences
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| PubMed ID |
1731341 |
| PubMed Abstract |
The Drosophila sequence-specific DNA binding protein, Adf-1, is capable of activating transcription of the alcohol dehydrogenase
gene, Adh, and is implicated in the transcriptional control of other developmentally regulated genes. We have cloned the cDNA
encoding Adf-1 by generating specific DNA probes deduced from partial amino acid sequence of the protein. Several cDNA clones
encoding an extended open reading frame were isolated from a phage lambda library. The complete amino acid sequence of Adf-1
deduced from the longest cDNA reveals structural similarities to the putative helix-turn-helix DNA binding motif of Myb and
Myb-related proteins. DNA sequence analysis of genomic clones and Northern blot analysis of mRNA suggest that Adf-1 is a single-copy
gene encoding a 1.9-kb transcript. Purified recombinant Adf-1 expressed in Escherichia coli binds specifically to Adf-1 recognition
sites and activates transcription of a synthetic Adh promoter in vitro in a manner indistinguishable from the protein purified
from Drosophila. Temporally staged Drosophila embryos immunochemically stained with affinity-purified anti-Adf-1 antibodies
indicate that Adf-1 protein is not detectable in very early embryos and does not appear to be maternally inherited. During
later stages of embryogenesis, Adf-1 appears to be expressed in the nucleus of most somatic cells in the embryo with possibly
higher concentrations found in some tissues.
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| Biosis |
93076517 |
| Zoological record |
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Associated Information
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| Text of personal communication |
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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| Place of publication |
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Published In
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| Abbreviation |
Proc. Natl. Acad. Sci. USA |
| Title |
Proceedings of the National Academy of Sciences of the United States of America |
| Authors |
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| Volume range |
1- |
| Year range |
1915- |
| Page range |
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| Publisher |
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| Place of publication |
Washington, DC |
| Language of publication |
English |
| ISBN/ISSN |
0027-8424 |
| CODEN |
PNASA6 |
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Data from Reference
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| Genes (1)
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