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Reference
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| Citation |
Lane, M.E., Kalderon, D. (1993). Genetic investigation of cAMP-dependent protein kinase function in Drosophila development. Genes Dev. 7(7A): 1229--1243. |
| FlyBase ID |
FBrf0058516 |
| Type of publication |
Research paper |
| Offprint Available |
No |
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External Crossreferences
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| PubMed ID |
8391504 |
| PubMed Abstract |
The cAMP-dependent protein kinase (PKA) has been shown to mediate the vast majority of cellular responses to the intracellular
second messenger, cAMP, in eukaryotes. To study the role of cAMP signal transduction in Drosophila development, we have isolated
and molecularly characterized mutations of varying severity in the Drosophila PKA gene, DC0. Biochemical measurements indicate
that DC0 is either the sole or the major PKA catalytic subunit gene in Drosophila. Adult females heterozygous for a strong
and a weak DC0 allele fail to lay eggs and show a striking and novel defect in oogenesis that includes the formation of egg
chambers containing multinucleate nurse cells. Females heterozygous for two weak DC0 alleles are fertile but produce offspring
showing a variety of defects in embryogenesis, including preblastoderm arrest and alterations in cuticular patterning. Animals
zygotically null for DC0 die as morphologically normal first-instar larvae, implying that maternally encoded protein, which
perdures for at least 12 hr, suffices for embryogenesis. Animals hemizygous for weak DC0 alleles survive for several days
as larvae but grow slowly. Mitotic recombination experiments in the adult eye indicate that the DC0 gene is not required autonomously
either for cell viability or normal growth rates. These results argue that cAMP-mediated signal transduction is essential
at a variety of stages during the development of a metazoan.
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| Biosis |
96075135 |
| Zoological record |
13002008064 |
Associated Information
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| Text of personal communication |
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Related Publications
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Also Published As
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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Published In
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| Abbreviation |
Genes Dev. |
| Title |
Genes & Development |
| Authors |
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| Volume range |
1- |
| Year range |
1987- |
| Page range |
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| Publisher |
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| Place of publication |
Cold Spring Harbor, NY |
| Language of publication |
English |
| ISBN/ISSN |
0890-9369 |
| CODEN |
GEDEEP |
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Data from Reference
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| Aberrations (23)
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| Alleles (13)
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| Constructs (1)
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| Genes (14)
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| Insertions (3)
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