A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Reference Report

Reference
Citation Zhang, P., Spradling, A.C. (1993). Efficient and dispersed local P-element transposition from Drosophila females.  Genetics 133(2): 361--373.
FlyBase ID FBrf0058565
Type of publication Research paper
Offprint Available Yes
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PubMed ID 8382178
PubMed Abstract We have investigated how Drosophila P element insertions are distributed in the chromosomal region near their starting site. A single P element residing in the euchromatin of minichromosome Dp1187 was mobilized following a cross to the delta 2-3 (99B) strain, and progeny bearing transpositions were identified with a minimum of bias by performing Southern blots on progeny. Approximately 1-2% of all progeny minichromosomes contained new insertions. Many of these "local transpositions" landed very close to or within the starting P element; however, nearly 1% of all progeny chromosomes contained new insertions 1-180 kb from the donor element. More local insertions were observed in the progeny of females than from male parents, and most occurred in a preferred orientation relative to the starting element. These observations suggested that donor elements are frequently excised and reinserted locally without ever dissociating from a transposition complex. The high frequency and diverse distribution of local transpositions recovered from females suggested that the efficiency of insertional mutagenesis can be significantly enhanced by using a starting P element(s) located near the target of interest.
Biosis 95096465
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Language of publication English
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Abbreviation Genetics
Title Genetics
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Volume range 1-
Year range 1916-
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Place of publication Austin, TX, etc
Language of publication English
ISBN/ISSN 0016-6731
CODEN GENTAE
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hideAberrations (19)
hideAlleles (3)
hideBalancers (1)
hideGenes (6)
hideInsertions (39)
hideNatural transposons (1)