Reference Report

Reference
Citation	Fujimoto, K., Masuda, K.I., Asada, N., Ohnishi, E. (1993). Purification and characterization of prophenoloxidases from pupae of Drosophila melanogaster. J. Biochem., Tokyo 113(3): 285--291. (Export to RIS)
FlyBase ID	FBrf0058767
Publication Type	Research paper
PubMed ID	8486601
PubMed Abstract	Two isoforms of prophenoloxidase were isolated from pupae of Oregon-R strain of Drosophila melanogaster. The purification procedure included ammonium sulfate fractionation, Sephacryl S-200 gel chromatography, DEAE-cellulose, and hydroxylapatite column chromatography. The two isoforms, A1 and A3, could be separated by ammonium sulfate fractionation. The isoelectric points of A1 and A3 were determined to be pH 5.8 and 6.7, respectively. The molecular weights of the monomers of A1 and A3 were estimated by SDS-PAGE to be 78 and 77 kDa, respectively. The native states of A1 and A3 are considered to be homodimeric, as judged by gel-filtration chromatography.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Biochem., Tokyo
Title	Journal of Biochemistry
Publication Year	1922-
ISBN/ISSN	0021-924X
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