Reference Report

Reference
Citation	McLean, C., Bucheton, A., Finnegan, D.J. (1993). The 5' untranslated region of the I factor, a long interspersed nuclear element-like retrotransposon of Drosophila melanogaster, contains an internal promoter and sequences that regulate expression. Mol. Cell. Biol. 13(2): 1042--1050. (Export to RIS)
FlyBase ID	FBrf0059029
Publication Type	Research paper
PubMed ID	8380889
PubMed Abstract	The I-R system of hybrid dysgenesis in Drosophila melanogaster is controlled by a long interspersed nuclear element-like retroposon, the I factor. Transposition of the I factor occurs at a high frequency only in the ovaries of females produced by crossing males of inducer strains that contain functional I factors with females of reactive strains that lack them. In this study, the 5' untranslated region of the I factor was joined to the chloramphenicol acetyltransferase gene, and activity was assayed in transfected D. melanogaster tissue culture cells and transformed flies. The results have identified a promoter that lies within the first 186 pb of the I factor. Deletion analysis shows that nucleotides +1 to +40 are sufficient for high promoter activity and accurate transcription initiation. This region contains sequences that are found in a class of RNA polymerase II promoters that lack both a TATA box and CpG-rich motifs. In transformed flies, high levels of expression from nucleotides +1 to +186 are confined to the ovaries of reactive females, suggesting that the promoter is involved in the tissue and cytotype specificity of transposition.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Cell. Biol.
Title	Molecular and Cellular Biology
Publication Year	1981-
ISBN/ISSN	0270-7306
Data from Reference
Alleles (13)
	Ecol\CAT^C4 Ecol\CAT^I-element.12 Ecol\CAT^I-element.28 Ecol\CAT^I-element.40 Ecol\CAT^{I-element.40G} Ecol\CAT^{I-element.40Δ4} Ecol\CAT^{I-element.40λ} Ecol\CAT^{I-element.100} Ecol\CAT^{I-element.186} Ecol\CAT^{I-element.186G} Ecol\CAT^{I-element.186Δ4.1} Ecol\CAT^{I-element.186Δ4.2} w^+mW.hs
Constructs (3)
	P{CAT^{I-element.186}} P{CATW8} pP{CATW8}
Genes (3)
	Ecol\CAT I-element\RTase w
Natural transposons (1)
	I-element