Reference Report

Reference
Citation	Brand, A.H., Perrimon, N. (1993). Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118(2): 401--415. (Export to RIS)
FlyBase ID	FBrf0064375
Publication Type	Research paper
PubMed ID	8223268
PubMed Abstract	We have designed a system for targeted gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns. The gene encoding the yeast transcriptional activator GAL4 is inserted randomly into the Drosophila genome to drive GAL4 expression from one of a diverse array of genomic enhancers. It is then possible to introduce a gene containing GAL4 binding sites within its promoter, to activate it in those cells where GAL4 is expressed, and to observe the effect of this directed misexpression on development. We have used GAL4-directed transcription to expand the domain of embryonic expression of the homeobox protein even-skipped. We show that even-skipped represses wingless and transforms cells that would normally secrete naked cuticle into denticle secreting cells. The GAL4 system can thus be used to study regulatory interactions during embryonic development. In adults, targeted expression can be used to generate dominant phenotypes for use in genetic screens. We have directed expression of an activated form of the Dras2 protein, resulting in dominant eye and wing defects that can be used in screens to identify other members of the Dras2 signal transduction pathway.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (22)
	Ecol\lacZ^Scer\UAS.cBa Ecol\lacZ^Scer\UAS.cFa eve^Scer\UAS.cBa h^1J3 l(1)BP¹ Ras64B^Val14.hs Ras64B^{Val14.Scer\UAS} Scer\GAL4^30A Scer\GAL4^32B Scer\GAL4^69B Scer\GAL4^71B Scer\GAL4^h-1J3 Scer\GAL4^how-24B Scer\GAL4^hs.2sev Scer\GAL4^l(1)BP-1 Scer\GAL4^{l(3)31-1-31-1} Scer\GAL4^prd.RG1 Scer\GAL4^Rh2.PB Scer\GAL4^wB Scer\UAS^5x.hs.SV40 w^+mC w^+mW.hs
Constructs (38)
	5xUAS 001151 001152 001153 001154 001158 001159 001186 001187 001188 001192 001260 Car1_0.1(H3,u) Car1_P5'(P5',u) GAL4::Hsp70Bb GAL4⁺2.9(r,H3) GawB_P5'(P5',Bg2) Hsp70Bb_P250(u,Xm) Hsp70Bb::lacZ::SV40_4.6 Hsp70Bb::SV40(uast) P{GAL4-Hsp70.sev} P{GAL4-prd.F} P{GawB} P{Rh2-GAL4.B} P{UAS-eve.B} P{UAS-lacZ.B} P{UAS-Ras64B.V14} P{UAST} P{Ubx-lacZ.-3.1} pGaTB pGaTN pP{GAL4-Rh2.B} pP{GawB} pP{UAS-eve.B} pP{UAS-lacZ.B} pP{UAS-Ras64B.V14} pP{UAST} uast.mcs
Genes (9)
	Ecol\lacZ eve h l(1)BP Ras64B Scer\GAL4 Scer\UAS w wg
Insertions (8)
	P{GawB}30A P{GawB}32B P{GawB}69B P{GawB}71B P{GawB}h^1J3 P{GawB}how^24B P{GawB}l(1)BP¹ P{GawB}l(3)31-1^31-1
Transcripts (1)
	Scer\GAL4^wBRA