Reference Report

Reference
Citation	Gremke, L., Lord, P.C., Sabacan, L., Lin, S.C., Wohlwill, A., Storti, R.V. (1993). Coordinate regulation of Drosophila tropomyosin gene expression is controlled by multiple muscle-type-specific positive and negative enhancer elements. Dev. Biol. 159(2): 513--527. (Export to RIS)
FlyBase ID	FBrf0064482
Publication Type	Research paper
PubMed ID	8405675
PubMed Abstract	Muscle development involves the coordinated regulation of transcription of muscle-type-specific genes and their encoded proteins during myogenesis. We show here that transcriptional regulation of the Drosophila tropomyosin I (TmI) gene during myogenesis is under the control of at least two muscle enhancer regions located within the first intron of the gene. Together these enhancer regions contain multiple muscle-type-specific positive and negative cis-acting elements which together contribute toward full expression of the gene. One of these enhancers is contained within a 355-bp fragment that is sufficient to direct high levels of temporally regulated expression from a heterologous promoter in all muscles of transgenic flies. Dissection of this enhancer region into smaller fragments has allowed us to identify a 91-bp enhancer fragment sufficient for directing expression in all somatic and visceral muscles of the larva and adult but not in the indirect flight muscles and tergal depressor of the trochanter or jump muscles of the adult. We also show that this somatic/visceral muscle element(s) can be repressed through an adjacent negative control region, suggesting that the regulation of expression in these muscles is under dual control during both phases of myogenesis. We propose a model in which transcriptional regulation of the Drosophila TmI gene is controlled by the cooperative interaction of multiple positive and negative cis-acting regulatory elements that control the temporal and muscle-type pattern of expression. The distribution of enhancer elements and their control of TmI gene expression are similar to those regulating transcription of the muscle promoter of the TmII gene and provide a framework for the coordinate expression of the two genes.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Alleles (15)
	Ecol\lacZ^hs.PS Ecol\lacZ^Tm2.1B.hs Ecol\lacZ^Tm2.1Ba.hs Ecol\lacZ^Tm2.1Ba+b.hs Ecol\lacZ^Tm2.1Bb.hs Ecol\lacZ^Tm2.1Bb+c.hs Ecol\lacZ^Tm2.1Bc.hs Ecol\lacZ^Tm2.A Ecol\lacZ^Tm2.B.hs Ecol\lacZ^Tm2.C.hs Ecol\lacZ^Tm2.D.hs Ecol\lacZ^Tm2.E.hs Ecol\lacZ^Tm2.F.hs Ecol\lacZ^Tm2.G.hs w^+mW.hs
Constructs (14)
	P{clone1Bb} P{Tm2(1B)lacZ} P{Tm2(1Ba)lacZ} P{Tm2(1Ba+b)lacZ} P{Tm2(1Bb+c)lacZ} P{Tm2(1Bc)lacZ} P{Tm2(A)lacZ} P{Tm2(B)lacZ} P{Tm2(C)lacZ} P{Tm2(D)lacZ} P{Tm2(E)lacZ} P{Tm2(F)lacZ} P{Tm2(G)lacZ} P{Whsp70}
Genes (4)
	Ecol\lacZ Tm1 Tm2 w
Sequence features (4)
	Tm2_intronI1B(a) Tm2_intronIC Tm2_intronID Tm2_intronIE
Transcripts (2)
	Ecol\lacZ^Tm2.B.hsRA Ecol\lacZ^Tm2.D.hsRA