Reference Report

Reference
Citation	Sosnowski, B.A., Davis, D.D., Boggs, R.T., Madigan, S.J., McKeown, M. (1994). Multiple portions of a small region of the Drosophila transformer gene are required for efficient in vivo sex-specific regulated RNA splicing and in vitro sex-lethal binding. Dev. Biol. 161(1): 302--312. (Export to RIS)
FlyBase ID	FBrf0068695
Publication Type	Research paper
PubMed ID	8293881
PubMed Abstract	The transformer gene of Drosophila is regulated by Sex-lethal-dependent 3' splice site blockage. 40 nucleotides immediately upstream of the regulated splice site are sufficient to direct sex-specific regulated splicing in transgenic animals. This entire region appears to be necessary for regulation and for efficient Sex-lethal binding. Natural splice sites containing partial homology to transformer do not show regulation. Mutations which replace the 16 nucleotides surrounding the branch point or alter single nucleotides near the splice site eliminate or reduce regulation without eliminating splicing. Mutations which reduce or eliminate regulation in vivo reduce binding to Sex-lethal in vitro, consistent with the hypothesis that these mutations bring about their effects by altering Sex-lethal binding rather than by altering binding sites for additional non-Sex-lethal factors.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Alleles (15)
	Adh^tra.rss Hsim\TK^{tra.Adhconstra} Hsim\TK^tra.AdhT8C Hsim\TK^tra.rss tra^M3 tra^M4 tra^M5 tra^M6 tra^M7 tra^M8 tra^M9 tra^M10 tra^M11 tra^M12 tra^unspecified
Constructs (14)
	P{Adh-tra} P{M10} P{M11} P{M12} P{TK-Adh-cons-tra} P{TK-Adh-T8C} P{TK-tra} P{traM3} P{traM4} P{traM5} P{traM6} P{traM7} P{traM8} P{traM9}
Genes (4)
	Adh Hsim\TK Sxl tra