Reference Report

Reference
Citation	Halter, D.A., Urban, J., Rickert, C., Ner, S.S., Ito, K., Travers, A.A., Technau, G.M. (1995). The homeobox gene repo is required for the differentiation and maintenance of glia function in the embryonic nervous system of Drosophila melanogaster. Development 121(2): 317--332. (Export to RIS)
FlyBase ID	FBrf0076135
Publication Type	Research paper
PubMed ID	7768175
PubMed Abstract	We describe the cloning, expression and phenotypic characterisation of repo, a gene from Drosophila melanogaster that is essential for the differentiation and maintenance of glia function. It is not, however, required for the initial determination of glial cells. In the embryo, the gene, which encodes a homeodomain protein, is expressed exclusively in all developing glia and closely related cells in both the central and peripheral nervous systems. The only observed exceptions in the CNS are the midline glia derived from the mesectoderm and two of three segmental nerve root glial cells. Using a polyclonal antibody we traced the spatial and temporal pattern of the protein expression in detail. Embryos homozygous for null alleles of the protein exhibit late developmental defects in the nervous system, including a reduction in the number of glial cells, disrupted fasciculation of axons, and the inhibition of ventral nerve cord condensation. The expression of an early glial-specific marker is unaffected in such homozygotes. By contrast, the expression of late glial-specific markers is either substantially reduced or absent. The specificity of expression is also observed in the locust Schistocerca gregaria and is thus evolutionarily conserved.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (1)
	In(3LR)TM3
Alleles (12)
	Ecol\lacZ⁰³⁵⁶³ Ecol\lacZ^gcm-rA87 Ecol\lacZ^Kr.PH3.7 Ecol\lacZ^M84 Ecol\lacZ^repo-00692 Ecol\lacZ^repo-02138 Ecol\lacZ^repo-03702 repo⁰⁰⁶⁹² repo⁰²¹³⁸ repo⁰³⁷⁰² repo^EX52 repo^EX84
Balancers (1)
	TM3-vSb
Constructs (1)
	P{Kr-lacZPH3.7}
Genes (3)
	Ecol\lacZ l(2)03563 repo
Insertions (4)
	P{PZ}l(2)03563⁰³⁵⁶³ P{PZ}repo⁰⁰⁶⁹² P{PZ}repo⁰²¹³⁸ P{PZ}repo⁰³⁷⁰²