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Citation
Balles, J., Pflugfelder, G.O. (1994). Facilitated isolation of rare recombinants by ligase chain reaction: Selection for intragenic crossover events in the Drosophila optomotor-blind gene.  Mol. Gen. Genet. 245(6): 734--740.
FlyBase ID
FBrf0078013
Publication Type
Research paper
Abstract

Ligase chain reaction (LCR) was evaluated as a tool for the detection of point mutations. For the mutation studied, the specificity of the method is sufficient to detect the mutant allele in the presence of a 200-fold molar excess of the wild-type sequence. LCR was therefore employed in a genetic recombination experiment as a probe for a recessive lethal point mutation. LCR greatly facilitated the isolation of a rare recombinant originating from a crossover event in the 40 kb interval separating the lethal mutation and an enhancer trap insertion in the optomotor-blind locus. The recombinant will allow the study of gene control in situ, in a largely unperturbed regulatory environment.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Mol. Gen. Genet.
    Title
    Molecular and General Genetics
    Publication Year
    1967-2001
    ISBN/ISSN
    0026-8925
    Data From Reference
    Aberrations (4)
    Alleles (5)
    Genes (2)
    Insertions (1)