Reference Report

Reference
Citation	Harrison, D.A., Binari, R., Nahreini, T.S., Gilman, M., Perrimon, N. (1995). Activation of a Drosophila Janus kinase (JAK) causes hematopoietic neoplasia and developmental defects. EMBO J. 14(12): 2857--2865. (Export to RIS)
FlyBase ID	FBrf0082087
Publication Type	Research paper
PubMed ID	7796812
PubMed Abstract	In mammals, many cytokines and growth factors stimulate members of the Janus kinase (JAK) family to transduce signals for the proliferation and differentiation of various cell types, particularly in hematopoietic lineages. Mutations in the Drosophila hopscotch (hop) gene, which encodes a JAK, also cause proliferative defects. Loss-of-function alleles result in lethality and underproliferation of diploid tissues of the larva. A dominant gain-of-function allele, Tumorous-lethal (hopTum-l), leads to formation of melanotic tumors and hypertrophy of the larval lymph glands, the hematopoietic organs. We show that a single amino acid change in Hop is associated with the hopTum-l mutation. Overexpression of either wild-type hop or hopTum-l in the larval lymph glands causes melanotic tumors and lymph gland hypertrophy indistinguishable from the original hopTum-l mutation. In addition, overexpression of Hop in other tissues of the larva leads to pattern defects in the adult or to lethality. Finally, overexpression of either hop or hopTum-l in Drosophila cell culture results in tyrosine phosphorylation of Hop protein. However, overexpression of hopTum-l results in greater phosphorylation than overexpression of the wild-type. We conclude that hopTum-l encodes a hyperactive Hop kinase and that overactivity of Hop in lymph glands causes malignant neoplasia of Drosophila blood cells.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	EMBO J.
Title	The EMBO Journal
Publication Year	1982-
ISBN/ISSN	0261-4189
Data from Reference
Alleles (35)
	hop^Act5C.PH hop^hs.PH hop^r1 hop^r2 hop^r3 hop^r4 hop^r5 hop^Scer\UAS.cHa hop^Tum.Act5C hop^Tum.hs hop^Tum.Scer\UAS hop^Tum Scer\GAL4^76B Scer\GAL4^c135 Scer\GAL4^c273 Scer\GAL4^c355 Scer\GAL4^c564 Scer\GAL4^c591 Scer\GAL4^c729 Scer\GAL4^c736 Scer\GAL4^c754 Scer\GAL4^e8A Scer\GAL4^e13C Scer\GAL4^e33C Scer\GAL4^en-e16E Scer\GAL4^hs.PB Scer\GAL4^T13 Scer\GAL4^T32 Scer\GAL4^T59 Scer\GAL4^T76 Scer\GAL4^T98 Scer\GAL4^T100 Scer\GAL4^T110 Scer\GAL4^T155 w^+mC
Constructs (11)
	P{en2.4-GAL4} P{GAL4-dpp.blk1} P{GAL4-Hsp70.PB} P{hs-hop} P{hs-Tum} P{UAS-hop.H} P{UAS-hop.Tum} pP{hs-hop} pP{hs-Tum} pP{UAS-hop.H} pP{UAS-hop.Tum}
Genes (3)
	hop Scer\GAL4 w
Insertions (21)
	P{en2.4-GAL4}e8A P{en2.4-GAL4}e13C P{en2.4-GAL4}e16E P{en2.4-GAL4}e33C P{GawB}76B P{GawB}c135 P{GawB}c273 P{GawB}c355 P{GawB}c564 P{GawB}c591 P{GawB}c729 P{GawB}c736 P{GawB}c754 P{GawB}l(2)T32^T32 P{GawB}l(2)T76^T76 P{GawB}T13 P{GawB}T59 P{GawB}T98 P{GawB}T100 P{GawB}T110 P{GawB}T155