|
Reference
|
| Citation |
Harrison, D.A., Binari, R., Nahreini, T.S., Gilman, M., Perrimon, N. (1995). Activation of a Drosophila Janus kinase (JAK) causes hematopoietic neoplasia and developmental defects. EMBO J. 14(12): 2857--2865.
|
| FlyBase ID |
FBrf0082087 |
| Type of publication |
Research paper |
| Offprint Available |
Yes |
|
External Crossreferences
|
| PubMed ID |
7796812 |
| PubMed Abstract |
In mammals, many cytokines and growth factors stimulate members of the Janus kinase (JAK) family to transduce signals for
the proliferation and differentiation of various cell types, particularly in hematopoietic lineages. Mutations in the Drosophila
hopscotch (hop) gene, which encodes a JAK, also cause proliferative defects. Loss-of-function alleles result in lethality
and underproliferation of diploid tissues of the larva. A dominant gain-of-function allele, Tumorous-lethal (hopTum-l), leads
to formation of melanotic tumors and hypertrophy of the larval lymph glands, the hematopoietic organs. We show that a single
amino acid change in Hop is associated with the hopTum-l mutation. Overexpression of either wild-type hop or hopTum-l in the
larval lymph glands causes melanotic tumors and lymph gland hypertrophy indistinguishable from the original hopTum-l mutation.
In addition, overexpression of Hop in other tissues of the larva leads to pattern defects in the adult or to lethality. Finally,
overexpression of either hop or hopTum-l in Drosophila cell culture results in tyrosine phosphorylation of Hop protein. However,
overexpression of hopTum-l results in greater phosphorylation than overexpression of the wild-type. We conclude that hopTum-l
encodes a hyperactive Hop kinase and that overactivity of Hop in lymph glands causes malignant neoplasia of Drosophila blood
cells.
|
| Biosis |
98409214 |
| Zoological record |
|
Associated Information
|
| Comments |
|
| Text of personal communication |
|
| Associated files |
|
Related Publications
|
|
|
Also Published As
|
|
|
Other Reference Information
|
| Secondary IDs |
|
| Language of publication |
English |
| Additional language(s) of abstract |
|
| ISBN |
|
| Place of publication |
|
Published In
|
| Abbreviation |
EMBO J. |
| Title |
The EMBO Journal |
| Authors |
|
| Volume range |
1- |
| Year range |
1982- |
| Page range |
|
| Publisher |
|
| Place of publication |
Oxford |
| Language of publication |
English |
| ISBN/ISSN |
0261-4189 |
| CODEN |
EMJODG |
Data from Reference
|
Alleles (35)
|
|
|
Constructs (11)
|
|
|
Genes (3)
|
|
|
Insertions (21)
|
|
|
|