Reference Report

Reference
Citation	Raabe, T., Olivier, J.P., Dickson, B., Liu, X., Gish, G.D., Pawson, T., Hafen, E. (1995). Biochemical and genetic analysis of the Drk SH2/SH3 adaptor protein of Drosophila. EMBO J. 14(11): 2509--2518. (Export to RIS)
FlyBase ID	FBrf0082502
Publication Type	Research paper
PubMed ID	7781603
PubMed Abstract	The Drk SH3-SH2-SH3 adaptor protein has been genetically identified in a screen for rate-limiting components acting downstream of the Sevenless (Sev) receptor tyrosine kinase in the developing eye of Drosophila. It provides a link between the activated Sev receptor and Sos, a guanine nucleotide release factor that activates Ras1. We have used a combined biochemical and genetic approach to study the interactions between Sev, Drk and Sos. We show that Tyr2546 in the cytoplasmic tail of Sev is required for Drk binding, probably because it provides a recognition site for the Drk SH2 domain. Interestingly, a mutation at this site does not completely block Sev function in vivo. This may suggest that Sev can signal in a Drk-independent, parallel pathway or that Drk can also bind to an intermediate docking protein. Analysis of the Drk-Sos interaction has identified a high affinity binding site for Drk SH3 domains in the Sos tail. We show that the N-terminal Drk SH3 domain is primarily responsible for binding to the tail of Sos in vitro, and for signalling to Ras in vivo.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	EMBO J.
Title	The EMBO Journal
Publication Year	1982-
ISBN/ISSN	0261-4189
Data from Reference
Alleles (13)
	drk^hs.sev drk^R1 drk^{SH3(N)SH2.hs.sev} drk^W36A.hs.sev drk^W189A.hs.sev drk^ΔP24 sev::tor^{13D:K2242M.hs.sev} sev::tor^{13D:Y2546F.hs.sev} sev::tor^13D.hs.sev sev¹⁴ sev^{Y1485A.W1486A.sev} sev^{Y1485A.W1486A.Y2546F.sev} w^+mW.hs
Constructs (9)
	P{sE-drk^W36A} P{sE-drk^W189A} P{sE-drk^wt} P{sE-sev^{Y1485A.W1486A.Y2546F}} P{sE-sev^{Y1485A.W1486A}} P{sE-torso⁴⁰²¹-sev} P{sE-torso⁴⁰²¹-sev^K2242M} P{sE-torso⁴⁰²¹-sev^Y2546F} P{SH3(N)SH2}
Genes (7)
	drk Ras85D sev sev::tor Sos tor w