Reference Report

Reference
Citation	Wodarz, A., Hinz, U., Engelbert, M., Knust, E. (1995). Expression of crumbs confers apical character on plasma membrane domains of ectodermal epithelia of Drosophila. Cell 82(1): 67--76. (Export to RIS)
FlyBase ID	FBrf0082789
Publication Type	Research paper
PubMed ID	7606787
PubMed Abstract	The crumbs protein of Drosophila is an integral membrane protein, with 30 EGF-like and 4 laminin A G domain-like repeats in its extracellular segment, which is expressed on the apical plasma membrane of all ectodermally derived epithelia. Here, we present evidence to show that the insertion of crumbs into the plasma membrane is necessary and sufficient to confer apical character on a membrane domain. Overexpression of crumbs results in an enormous expansion of the apical plasma membrane and the concomitant reduction of the basolateral domain. This is followed by the redistribution of beta Heavy-spectrin, a component of the membrane cytoskeleton, and by the ectopic deposition of cuticle and other apical components into these areas. Strikingly, overexpression of the membrane-bound cytoplasmic portion of crumbs alone is sufficient to produce this dominant phenotype. Our results suggest that crumbs plays a key role in specifying the apical plasma membrane domain of ectodermal epithelial cells of Drosophila.
DOI
Related Publication(s)
Review	Monitor. Anonymous, 1995, Trends Genet. 11(10): 387 [FBrf0091276]
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Secondary IDs
Language of Publication	English
Additional Languages of Abstract
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Parent Publication
Publication Type	Journal
Abbreviation	Cell
Title	Cell
Publication Year	1974-
ISBN/ISSN	0092-8674
Data from Reference
Alleles (11)
	crb^11A22 crb^{extra.Scer\UAS} crb^{intra.Scer\UAS.T:Hsap\MYC} crb^{intra.Scer\UAS} crb^Scer\UAS.cWa Scer\GAL4^332.3 Scer\GAL4^385.3 Scer\GAL4^da.G32 Scer\GAL4^ptc-559.1 w^+mC w^+mW.hs
Constructs (12)
	P{GAL4-da.G32} P{UAS-crb.extra} P{UAS-crb.intra} P{UAS-crb.myc} P{UAS-crb.wt} P{W5N} pP{GAL4-da.G32} pP{UAS-crb.extra} pP{UAS-crb.intra} pP{UAS-crb.myc} pP{UAS-crb.wt} pP{W5N}
Genes (9)
	crb Fas3 ptc sas Scer\GAL4 sdt T:Hsap\MYC w β-Spec
Insertions (9)
	P{GawB}332.3 P{GawB}385.3 P{GawB}ptc^559.1 P{UAS-crb.extra}34.1j13a P{UAS-crb.myc}2b P{UAS-crb.myc}38.14a P{UAS-crb.wt}1d P{UAS-crb.wt}2a P{UAS-crb.wt}4b