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Citation
Yeh, E., Gustafson, K., Boulianne, G.L. (1995). Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila.  Proc. Natl. Acad. Sci. U.S.A. 92(15): 7036--7040.
FlyBase ID
FBrf0082810
Publication Type
Research paper
Abstract

We have used the green fluorescent protein (GFP) from the jellyfish Aequorea victoria as a vital marker/reporter in Drosophila melanogaster. Transgenic flies were generated in which GFP was expressed under the transcriptional control of the yeast upstream activating sequence that is recognized by GAL4. These flies were crossed to several GAL4 enhancer trap lines, and expression of GFP was monitored in a variety of tissues during development using confocal microscopy. Here, we show that GFP could be detected in freshly dissected ovaries, imaginal discs, and the larval nervous system without prior fixation or the addition of substrates or antibodies. We also show that expression of GFP could be monitored in intact living embryos and larvae and in cultured egg chambers, allowing us to visualize dynamic changes in gene expression during real time.

PubMed ID
PubMed Central ID
PMC41466 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Proc. Natl. Acad. Sci. U.S.A.
    Title
    Proceedings of the National Academy of Sciences of the United States of America
    Publication Year
    1915-
    ISBN/ISSN
    0027-8424
    Data From Reference
    Alleles (12)
    Genes (4)
    Molecular Constructs (1)
    Insertions (12)
    Experimental Tools (2)
    Transgenic Constructs (2)