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Reference
Citation	Gomez-Skarmeta, J.L., Rodriguez, I., Martinez, C., Culi, J., Ferres-Marco, D., Beamonte, D., Modolell, J. (1995). Cis-regulation of achaete and scute: shared enhancer-like elements drive their coexpression in proneural clusters of the imaginal discs. Genes Dev. 9(15): 1869--1882.
FlyBase ID	FBrf0083155
Type of publication	Research paper
Offprint Available	Yes
External Crossreferences
PubMed ID	7649474
PubMed Abstract	The pattern of bristles and other sensory organs on the adult cuticle of Drosophila is prefigured in the imaginal discs by the pattern of expression of the proneural achaete (ac) and scute (sc) genes, two members of the ac-sc complex (AS-C). These genes are simultaneously expressed by groups of cells (the proneural clusters) located at constant positions in discs. Their products (transcription factors of the basic-helix-loop-helix family) allow cells to become sensory organ mother cells (SMCs), a fate normally realized by only one or a few cells per cluster. Here we show that the highly complex pattern of proneural clusters is constructed piecemeal, by the action on ac and sc of site-specific, enhancer-like elements distributed along most of the AS-C (approximately 90 kb). Fragments of AS-C DNA containing these enhancers drive reporter lacZ genes in only one or a few proneural clusters. This expression is independent of the ac and sc endogenous genes, indicating that the enhancers respond to local combinations of factors (prepattern). We show further that the cross-activation between ac and sc, discovered by means of transgenes containing either ac or sc promoter fragments linked to lacZ and thought to explain the almost identical patterns of ac and sc expression, does not occur detectably between the endogenous ac and sc genes in most proneural clusters. Our data indicate that coexpression is accomplished by activation of both ac and sc by the same set of position-specific enhancers.
Biosis	98467557
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Language of publication	English
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Abbreviation	Genes Dev.
Title	Genes & Development
Authors
Volume range	1-
Year range	1987-
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Place of publication	Cold Spring Harbor, NY
Language of publication	English
ISBN/ISSN	0890-9369
CODEN	GEDEEP
Data from Reference
Aberrations (10)
	Df(1)ase-1 Df(1)sc10-1 In(1)sc⁴ In(1)sc⁷ In(1)sc⁸ In(1)sc^8Lsc^4R In(1)sc⁹ In(1)y^3P In(1)y^3PLsc^8R T(1;2)sc^S2
Alleles (24)
	Ecol\lacZ^Hsp70Bb.HZ50 Ecol\lacZ^ac.0.8+5.7 Ecol\lacZ^ac.0.8+6.0 Ecol\lacZ^ac.0.8 Ecol\lacZ^sc.0.76 Ecol\lacZ^sc.3.7+0.6 Ecol\lacZ^sc.3.7+1.2 Ecol\lacZ^sc.3.7+3.1 Ecol\lacZ^sc.3.7+3.8 Ecol\lacZ^sc.3.7+5.7 Ecol\lacZ^sc.3.7+6.0 Ecol\lacZ^sc.3.7 ac³ ac^Hw-1 sc^10-1 sc⁴ sc⁶ sc⁷ sc⁸ sc⁹ sc^M6 sc^S2 su(Hw)² su(Hw)⁷
Constructs (11)
	P{0.8ac-lacZ} P{3.7sc-lacZ} P{ac/sc+0.6} P{ac/sc+0.76} P{ac/sc+1.2} P{ac/sc+3.1} P{ac/sc+3.8} P{ac/sc0.8+5.7} P{ac/sc0.8+6.0} P{ac/sc3.7+5.7} P{ac/sc3.7+6.0}
Genes (9)
	Cyp4g1 Ecol\lacZ ac ase l(1)sc pcl sc su(Hw) y
Insertions (1)
	gypsy{}ac^Hw-1
Transcripts (1)
	Ecol\lacZ^ac.0.8RA