Reference Report

Reference
Citation	Platero, J.S., Hartnett, T., Eissenberg, J.C. (1995). Functional analysis of the chromo domain of HP1. EMBO J. 14(16): 3977--3986. (Export to RIS)
FlyBase ID	FBrf0083372
Publication Type	Research paper
PubMed ID	7664737
PubMed Abstract	Heterochromatin protein 1 (HP1) is a non-histone chromosomal protein in Drosophila with dosage-dependent effects on heterochromatin-mediated gene silencing. An evolutionarily conserved amino acid sequence in the N-terminal half of HP1 (the 'chromo domain') shares > 60% sequence identity with a motif found in the Polycomb protein, a silencer of homeotic genes. We report here that point mutations in the HP1 chromo domain abolish the ability of HP1 to promote gene silencing. We show that the HP1 chromo domain, like the Polycomb chromo domain, has chromosome binding activity, but to distinct chromosomal sites. We constructed a chimeric HP1-Polycomb protein, consisting of the chromo domain of Polycomb in the context of HP1, and show that it binds to both heterochromatin and Polycomb binding sites in polytene chromosomes. In flies expressing chimeric HP1-Polycomb protein, endogenous HP1 is mislocalized to Polycomb binding sites, and endogenous polycomb is misdirected to the heterochromatic chromocenter, suggesting that both proteins are recruited to their distinct chromosomal binding sites through protein-protein contacts. Chimeric HP1-Polycomb protein expression in transgenic flies promotes heterochromatin-mediated gene silencing, supporting the view that the chromo domain homology reflects a common mechanistic basis for homeotic and heterochromatic silencing.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	EMBO J.
Title	The EMBO Journal
Publication Year	1982-
ISBN/ISSN	0261-4189
Data from Reference
Alleles (7)
	Pc::Su(var)205^{hs.T:Ecol\lacZ} Su(var)205² Su(var)205^{EEEAAA.hs.T:Ecol\lacZ} Su(var)205^{KQ.hs.T:Ecol\lacZ} Su(var)205^{NLS.hs.T:Ecol\lacZ} Su(var)205^{RRQQ.hs.T:Ecol\lacZ} Su(var)205^{YF.hs.T:Ecol\lacZ}
Constructs (6)
	P{EEE->AAA} P{HP1-Pc} P{K->Q} P{NLS} P{RR->QQ} P{Y->F}
Genes (4)
	Pc Pc::Su(var)205 Su(var)205 T:Ecol\lacZ