Reference Report

Reference
Citation	Roseman, R.R., Johnson, E.A., Rodesch, C.K., Bjerke, M., Nagoshi, R.N., Geyer, P.K. (1995). A P element containing suppressor of Hairy-wing binding regions has novel properties for mutagenesis in Drosophila melanogaster. Genetics 141(3): 1061--1074.
FlyBase ID	FBrf0084323
Type of publication	Research paper
Offprint Available	Yes
External Crossreferences
PubMed ID	8582613
PubMed Abstract	P elements are widely used as insertional mutagens to tag genes, facilitating molecular cloning and analyses. We modified a P element so that it carried two copies of the suppressor of Hairy-wing [su(Hw)] binding regions isolated from the gypsy transposable element. This transposon was mobilized, and the genetic consequences of its insertion were analyzed. Gene expression can be altered by the su(Hw) protein as a result of blocking the interaction between enhancer/silencer elements and their promoter. These effects can occur over long distances and are general. Therefore, a composite transposon (SUPor-P for suppressor-P element) combines the mutagenic efficacy of the gypsy element with the controllable transposition of P elements. We show that, compared to standard P elements, this composite transposon causes an expanded repertoire of mutations and produces alleles that are suppressed by su(Hw) mutations. The large number of heterochromatic insertions obtained is unusual compared to other insertional mutagenesis procedures, indicating that the SUPor-P transposon may be useful for studying the structural and functional properties of heterochromatin.
Biosis	98562391
Zoological record
Associated Information
Comments
Text of personal communication
Associated files
Related Publications

Also Published As

Other Reference Information
Secondary IDs
Language of publication	English
Additional language(s) of abstract
ISBN
Place of publication
Published In
Abbreviation	Genetics
Title	Genetics
Authors
Volume range	1-
Year range	1916-
Page range
Publisher
Place of publication	Austin, TX, etc
Language of publication	English
ISBN/ISSN	0016-6731
CODEN	GENTAE
Data from Reference
Aberrations (9)
	Df(2R)PC4 Df(3L)Ar14-8 Df(3L)rdgC-co2 Df(3R)CA3 Df(3R)awd-KRB Df(3R)ry27 In(1)sc⁸+dl-49 In(2LR)O In(3L)std11
Alleles (20)
	ct^546-1 ct⁶ f¹ gypsy\su(Hw)BR⁴³⁰ l(2)55BCa^274-2 l(2)55Da^411-1 l(2)55Db^425-1 l(3)100CDa^126M19 l(3)61Da^286-4 l(3)73CFa^82c l(3)85Ak^850-1 l(3)87Em^59-2 polo^16-1 su(Hw)⁷ su(Hw)^V w^+mET w^BR.BR w^BR.E.BR y^+mDint2 y^+t7.7
Balancers (3)
	CyO CyO-P{SUPor-P}RR1 FM0
Constructs (2)
	P{SUPor-P-lt} P{SUPor-P}
Genes (15)
	ct f gypsy\su(Hw)BR l(2)55BCa l(2)55Da l(2)55Db l(3)100CDa l(3)61Da l(3)73CFa l(3)85Ak l(3)87Em polo su(Hw) w y
Insertions (17)
	P{SUPor-P}316-1 P{SUPor-P}44a P{SUPor-P}450-1 P{SUPor-P}478-1 P{SUPor-P}50-2B P{SUPor-P}525-1 P{SUPor-P}RR1 P{SUPor-P}ct^546-1 P{SUPor-P}l(2)55BCa^274-2 P{SUPor-P}l(2)55Da^411-1 P{SUPor-P}l(2)55Db^425-1 P{SUPor-P}l(3)100CDa^126M19 P{SUPor-P}l(3)61Da^286-4 P{SUPor-P}l(3)73CFa^82c P{SUPor-P}l(3)85Ak^850-1 P{SUPor-P}l(3)87Em^59-2 P{SUPor-P}polo^16-1
Natural transposons (1)
	gypsy