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Citation
Stathakis, D.G., Pentz, E.S., Freeman, M.E., Kullman, J., Hankins, G.R., Pearlson, N.J., Wright, T.R.F. (1995). The genetic and molecular organization of the Dopa decarboxylase gene cluster of Drosophila melanogaster.  Genetics 141(2): 629--655.
FlyBase ID
FBrf0084402
Publication Type
Research paper
Abstract

We report the complete molecular organization of the Dopa decarboxylase gene cluster. Mutagenesis screens recovered 77 new Df(2L)TW130 recessive lethal mutations. These new alleles combined with 263 previously isolated mutations in the cluster to define 18 essential genes. In addition, seven new deficiencies were isolated and characterized. Deficiency mapping, restriction fragment length polymorphism (RFLP) analysis and P-element-mediated germline transformation experiments determined the gene order for all 18 loci. Genomic and cDNA restriction endonuclease mapping, Northern blot analysis and DNA sequencing provided information on exact gene location, mRNA size and transcriptional direction for most of these loci. In addition, this analysis identified two transcription units that had not previously been identified by extensive mutagenesis screening. Most of the loci are contained within two dense subclusters. We discuss the effectiveness of mutagens and strategies used in our screens, the variable mutability of loci within the genome of Drosophila melanogaster, the cytological and molecular organization of the Ddc gene cluster, the validity of the one band-one gene hypothesis and a possible purpose for the clustering of genes in the Ddc region.

PubMed ID
PubMed Central ID
PMC1206762 (PMC) (EuropePMC)
DOI
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference
    Aberrations (59)
    Alleles (402)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Genes (26)
    Insertions (1)
    Transgenic Constructs (25)