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Citation
Sun, Y.H., Tsai, C.J., Green, M.M., Chao, J.L., Yu, C.T., Jaw, T.J., Yeh, J.Y., Bolshakov, V.N. (1995). white as a reporter gene to detect transcriptional silencers specifying position-specific gene expression during Drosophila melanogaster eye development.  Genetics 141(3): 1075--1086.
FlyBase ID
FBrf0084424
Publication Type
Research paper
Abstract

The white+ gene was used as a reporter to detect transcriptional silencer activity in the Drosophila genome. Changes in the spatial expression pattern of white were scored in the adult eye as nonuniform patterns of pigmentation. Thirty-six independent P[lacW] transposant lines were collected. These represent 12 distinct pigmentation patterns and probably 21 loci. The spatial pigmentation pattern is due to cis-acting suppression of white+ expression, and the suppression probably depends on cell position rather than cell type. The mechanism of suppression differs from inactivation by heterochromatin. In addition, activation of lacZ in P[lacW] occurs also in specific patterns in imaginal discs and embryos in many of the lines. The expression patterns of white+ and lacZ may reflect the activity of regulatory elements belonging to an endogenous gene near each P[lacW] insertion site. We speculate that these putative POSE (position-specific expression) genes may have a role in pattern formation of the eye as well as other imaginal structures. Three of the loci identified are optomotor-blind, engrailed and invected. teashirt is also implicated as a candidate gene. We propose that this "silencer trap"' may be an efficient way of identifying genes involved in imaginal pattern formation.

PubMed ID
PubMed Central ID
PMC1206831 (PMC) (EuropePMC)
DOI
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference
    Aberrations (2)
    Alleles (80)
    Balancers (2)
    Genes (13)
    Insertions (37)
    Transgenic Constructs (1)