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Reference
Citation
Hacker, U., Kaufmann, E., Hartmann, C., Jurgens, G., Knochel, W., J├Ąckle, H. (1995). The Drosophila fork head domain protein crocodile is required for the establishment of head structures.  EMBO J. 14(21): 5306--5317.
FlyBase ID
FBrf0085156
Publication Type
Research paper
Abstract

The fork head (fkh) domain defines the DNA-binding region of a family of transcription factors which has been implicated in regulating cell fate decisions across species lines. We have cloned and molecularly characterized the crocodile (croc) gene which encodes a new family member from Drosophila. croc is expressed in the head anlagen of the blastoderm embryo under the control of the anterior, the dorsoventral and the terminal maternal organizer systems. The croc mutant phenotype indicates that the croc wild-type gene is required to function as an early patterning gene in the anterior-most blastoderm head segment anlage and for the establishment of a specific head skeletal structure that derives from the non-adjacent intercalary segment at a later stage of embryogenesis. As an early patterning gene, croc exerts unusual properties which do not allow it to be grouped among the established segmentation genes. A single-site mutation within the croc fkh domain, which causes a replacement of the first out of four conserved amino acid residues thought to be involved in the coordinate binding of Mg2+, abolishes the DNA binding of the protein in vitro. In view of the resulting lack-of-function mutant phenotype, it appears likely that metal binding by the affected region of the fkh domain is crucial for proper folding of the DNA-binding structure.

PubMed ID
PubMed Central ID
PMC394640 (PMC) (EuropePMC)
DOI
Associated Information
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    EMBO J.
    Title
    The EMBO Journal
    Publication Year
    1982-
    ISBN/ISSN
    0261-4189
    Data From Reference
    Aberrations (2)
    Alleles (15)
    Genes (17)
    Sequence Features (3)
    Insertions (1)
    Experimental Tools (1)
    Transgenic Constructs (4)
    Transcripts (3)