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Reference
Citation	Johnson, R.L., Grenier, J.K., Scott, M.P. (1995). patched overexpression alters wing disc size and pattern: transcriptional and post-transcriptional effects on hedgehog targets. Development 121(12): 4161--4170.
FlyBase ID	FBrf0085299
Type of publication	Research paper
Offprint Available	No
External Crossreferences
PubMed ID	8575316
PubMed Abstract	The membrane protein, Patched, plays a critical role in patterning embryonic and imaginal tissues in Drosophila. patched constitutively inactivates the transcription of target genes such as wingless, decapentaplegic, and patched itself. The secreted protein, Hedgehog, induces transcription of target genes by opposing the Patched signaling pathway. Using the Gal4 UAS system we have overexpressed patched in wing imaginal discs and found that high Patched levels, expressed in either normal or ectopic patterns, result in loss of wing vein patterning in both compartments centering at the anterior/posterior border. In addition, patched inhibits the formation of the mechanosensory neurons, the campaniform sensilla, in the wing blade. The patched wing vein phenotype is modulated by mutations in hedgehog and cubitus interruptus (ci). Patched overexpression inhibits transcription of patched and decapentaplegic and post-transcriptionally decreases the amount of Ci protein at the anterior/posterior boundary. In hedgehogMrt wing discs, which express ectopic hedgehog, Ci levels are correspondingly elevated, suggesting that hedgehog relieves patched repression of Ci accumulation. Protein kinase A also regulates Ci; protein kinase A mutant clones in the anterior compartment have increased levels of Ci protein. Thus patched influences wing disc patterning by decreasing Ci protein levels and inactivating hedgehog target genes in the anterior compartment.
Biosis	98680545
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Language of publication	English
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Abbreviation	Development
Title	Development
Authors
Volume range	99-
Year range	1987-
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Place of publication	Cambridge
Language of publication	English
ISBN/ISSN	0950-1991
CODEN	DEVPED
Data from Reference
Aberrations (2)
	Df(2R)P14TE Df(4)M101-62f
Alleles (14)
	Ecol\lacZ^Scer\UAS.cBa Ecol\lacZ^neur-A101 Ecol\lacZ^ptc-AT90 Scer\GAL4^71B Scer\GAL4^en-e16E Scer\GAL4^ptc-559.1 ci^Ce-1 cos^V1 hh^Mrt hh^Mrtr1 hh^Mrtr2 ptc^Scer\UAS.cJa ptc^tuf-1 w^+mC
Constructs (3)
	P{UAS-lacZ.B} P{UAS-ptc.J} pP{UAS-ptc.J}
Genes (8)
	Ecol\lacZ Pka-C1 Scer\GAL4 ci cos hh ptc w
Insertions (9)
	P{GawB}71B P{GawB}ptc^559.1 P{UAS-ptc.J}B1 P{UAS-ptc.J}B2 P{UAS-ptc.J}B3 P{UAS-ptc.J}C P{en2.4-GAL4}e16E P{lArB}neur^A101 P{lacZ-un2}ptc-AT90