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Citation
Minden, J.S. (1996). Synthesis of a new substrate for detection of lacZ gene expression in live Drosophila embryos.  BioTechniques 20(1): 122--129.
FlyBase ID
FBrf0085593
Publication Type
Research paper
Abstract

Escherichia coli lacZ, which encodes beta-galactosidase, has become a widely used reporter gene to study the developmental regulation of gene expression in a variety of organisms. To detect the presence of the beta-galactosidase, the sample must be fixed and appropriately stained. This sort of analysis yields rather crude estimates of the spatial-temporal changes in gene expression patterns. In addition, one cannot recover interesting specimens for propagation. A novel fluorogenic beta-galactosidase substrate for use in live Drosophila melanogaster embryos has been designed and synthesized. This compound provides a means to determine gene dosage in live embryos so that one can unambiguously determine the genotype of a living embryo. This will be useful for detailed analysis of cellular and morphogenetic behavior changes in live embryos that are homozygous for embryonic lethal mutations. In the course of testing this compound, a new beta-galactoside hydrolytic activity, different from the previously identified beta-galactosidase, has been discovered to reside in macrophages and the intervitelline space.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    BioTechniques
    Title
    BioTechniques
    Publication Year
    1983-
    ISBN/ISSN
    0736-6205
    Data From Reference
    Aberrations (3)
    Alleles (5)
    Balancers (2)
    Genes (2)
    Insertions (1)
    Transgenic Constructs (3)