A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Reference Report

Reference
Citation Dickson, B. (1996.3.29). Transgenic lines 1010T2 and 1010T10. 
FlyBase ID FBrf0086268
Type of publication Personal communication to FlyBase
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Text of personal communication
Date: Fri, 29 Mar 1996 11:00:00 +0100
To: Rachel Drysdale (Genetics) <rd120@XXXXXXXXXXXXXXX>
From: barry@XXXXXXXXXXXXXXX (Barry Dickson)
Dear Rachel,
In my nomenclature for transgenic lines, 1010T2 and 1010T10 represent
different insertions (T2 and T10) of the same plasmid (pBD1010). These
numbers are therefore quite meaningless to anyone but me, but since I have
unfortunately distributed these stocks to several people using these
designations, I guess they will have to stick.
Thanks, and best wishes,
Barry
>I am curating the genetic information about these constructs which you
>kindly deposited with Kath in the stock center. To be sure we get this
>right, could you confirm that 1010T[[2]] and 1010T[[10]] are insertion
>identifiers, as opposed to a description of something that is inside the
>transposon? (or otherwise set us straight!)
>
>> 1010T[[2]]: UAS-GFP[[S65T]], pCaSpeR3, II.chromosome homozygous viable
>> insert, construct and transgene done by Barry Dickson (unpublished)
>> using the GFP[[S65T]] mutant [Heim, R. and Tsien, R.Y. (1996) Current
>> Biology 6, 178-182].
>>
>> 1010T[[10]]: UAS-GFP[[S65T]], pCaSpeR3, III.chromosome homozygous viable
>> insert, construct and transgene done by Barry Dickson (unpublished)
>> using the GFP[[S65T]] mutant [Heim, R. and Tsien, R.Y. (1996) Current
>> Biology 6, 178-182].
>
>with best wishes,
>
>Rachel
>
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