Reference Report

Reference
Citation	Gustavson, E., Goldsborough, A.S., Ali, Z., Kornberg, T.B. (1996). The Drosophila engrailed and invected genes: Partners in regulation, expression and function. Genetics 142(3): 893--906. (Export to RIS)
FlyBase ID	FBrf0086432
Publication Type	Research paper
PubMed ID	8849895
PubMed Abstract	We isolated and characterized numerous engrailed and invected alleles. Among the deficiencies we isolated, a mutant lacking invected sequences was viable and phenotypically normal, a mutant lacking engrailed was an embryo lethal and had slight segmentation defects, and a mutant lacking both engrailed and invected was most severely affected. In seven engrailed alleles, mutations caused translation to terminate prematurely in the central or C-terminal portion of the coding sequence, resulting in embryonic lethality and segmentation defects. Both engrailed and invected expression declined prematurely in these mutant embryos. In wild-type embryos, engrailed and invected are juxtaposed and are expressed in essentially identical patterns. A breakpoint mutant that separates the engrailed and invected transcription units parceled different aspects of the expression pattern to engrailed or invected. We also found that both genes cause similar defects when expressed ectopically and that the protein products of both genes act to repress transcription in cultured cells. We propose that the varied phenotypes of the engrailed alleles can be explained by the differential effects these mutants have on the combination of engrailed and invected activities, that engrailed and invected share a regulatory region, and that they encode redundant functions.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (7)
	Df(2R)en^C Df(2R)en^E Df(2R)en^F Df(2R)en-SFX31 In(2LR)En(en)eg-1 In(2LR)O T(2;?)inv^l77
Alleles (47)
	Ecol\CAT^T3N6-B En(en)eg-1^eg-1 en¹ en² en³ en⁴ en⁶ en⁷ en⁸ en⁹ en³⁰ en³⁴ en³⁸ en⁴³ en⁴⁷ en⁴⁸ en⁴⁹ en⁵⁰ en⁵¹ en⁵² en⁵³ en⁵⁴ en⁵⁷ en⁵⁸ en⁵⁹ en^Act5C.PG en^C en^CX1 en^E en^Es en^F en^hs.PGa en^SF59 en^SF64 en^SF65 en^SF66 en^SF67 en^SF68 en^SF69 en^Xho25 inv³⁰ inv^Act5C.PG inv^E inv^hs.PG inv^l77 inv^Xba21 w^+mC
Balancers (1)
	CyO-ftz-lacZ
Constructs (2)
	P{HS-en.G} P{hsp70-inv}
Genes (7)
	ci Ecol\CAT en En(en)eg-1 hh inv w
Insertions (1)
	P{en-lacZ(wXba)}inv^Xba21