Reference Report

Reference
Citation	Wassarman, D.A., Solomon, N.M., Chang, H.C., Karim, F.D., Therrien, M., Rubin, G.M. (1996). Protein phosphatase 2A positively and negatively regulates Ras1-mediated photoreceptor development in Drosophila. Genes Dev. 10(3): 272--278. (Export to RIS)
FlyBase ID	FBrf0086675
Publication Type	Research paper
PubMed ID	8595878
PubMed Abstract	Protein phosphatase 2A (PP2A), a heterotrimeric serine/threonine phosphatase present in most tissues and cell types, has been implicated in the regulation of cell cycle progression, DNA replication, transcription, and translation. Here we present genetic evidence suggesting that PP2A functions downstream of Ras1 in the Sevenless receptor tyrosine kinase (RTK) signal transduction pathway that specifies R7 photoreceptor cell fate in the developing Drosophila eye. Ras1 and downstream cytoplasmic kinases, Raf, MEK, and MAPK, comprise an evolutionarily conserved cascade that mediates the transmission of signals from RTKs at the plasma membrane to specific factors in the nucleus. Using transgenic flies expressing constitutively activated Ras1 or Raf proteins that function independently of upstream signaling events, we show that a reduction in the dose of the gene encoding the catalytic subunit of PP2A stimulates signaling from Ras1 but impairs signaling from Raf. This suggests that PP2A both negatively and positively regulates the Ras1 cascade by dephosphorylating factors that function at different steps in the cascade.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Aberrations (17)
	Df(2L)XE-52 Df(2L)XE-715 Df(2L)XE-2267 Df(2L)XE-2269 Df(2L)XE-2387 Df(2L)XE-2519 Df(2L)XE-2668 Df(2L)XE-2750 Df(2L)XE-2794 Df(2L)XE-2860 Df(2L)XE-3103 Df(2L)XE-3494 Df(2L)XE-3595 Df(2L)XE-3696 Df(2L)XE-3781 Df(2L)XE-3801 Df(2L)XE-3841
Alleles (11)
	Ecol\CAT^Rh4.PF mts⁰²⁴⁹⁶ mts⁰⁵⁵⁵⁹ mts^k06009 mts^k12502 mts^k16501 mts^XE-2202 mts^XE-2258 phl::tor^13D.hs.sev phl¹² Ras85D^V12.sev
Constructs (3)
	P{Rh4.1900,CAT} P{sE-raf^tor4021} P{sevRas1.V12}
Genes (6)
	Ecol\CAT mts phl phl::tor Ras85D tor
Insertions (4)
	P{lacW}mts^k12502 P{lacW}mts^k16501 P{PZ}mts⁰²⁴⁹⁶ P{PZ}mts⁰⁵⁵⁵⁹