|Citation||Roote, J. (1996.5.20). 35B. (Export to RIS)|
|Publication Type||Personal communication to FlyBase|
|Text of Personal Communication||
From cesunkel@XXXXXXXXXXXXXXX Thu Jan 25 22:21:56 1996
Date: Thu, 25 Jan 96 22:52:30 PST
Reply-To: cesunkel@XXXXXXXXXXXXXXX (claudio sunkel)
From: cesunkel@XXXXXXXXXXXXXXX (claudio sunkel)
We have a P mutant that has a mitotic phenotype that is uncovered
both by Df(2L)A220 35B01-02;35B09 and Df(2L)35B01-03;35BC04-05.
The stock appears to have two insertions very close to one
another. One at 35B and the other at 35C. The one we are
interested is in 35B since that is lethal over Df(2L)A220 and has
a mitotic phenotype. We cloned one side of the insertion at 35B by
reverse PCR and have some 500 pb of sequence from a region some 1
kb away from the P-insertion. The reason why it is at some
distance from the end of the P is complicated but we are sure that
is the insertion site because it has internal P sequences as we
expected from a very rough idea of the transposon and also by in
situ using the cloned PCR product. These stocks
were made by J.M.Dura but I have not been able to find him. Any
way since we have the sequence I thought I could send you the P
stock as well as the sequence to compare against the sequence that
you have over the whole region. We have also isolated genomic
clones and are in the process of screening a cDNA library. If the
sequence matches somewhere in your sequence it would be great. It
might even be possible to find right away what kind of gene is
mutated. Can you do the search and let me know the results ??
Please let me know about all this and I will send you both the
sequence by email and the p-stock. I cannot send you the seq at
the moment because I am writing from home but I will do it
From cscariol@XXXXXXXXXXXXXXX Tue Jan 30 08:50:24 1996
Date: Tue, 30 Jan 1996 09:44:14 +0100
From: Claudio Enrique Sunkel Cariola <cscariol@XXXXXXXXXXXXXXX
To: M.ASHBURNERXXXXXXXXXXXXXXX, cscariolXXXXXXXXXXXXXXX
The reference for the p-element at 35B is #1356 but is not in any stock center.
It was part of a collection Kathy was going to throw away because they had not
The sequences I have are as follows:
These sequences are some 1100 bp from one of the ends of the insertion but
I do not know the orientation with respect to the chromocenter.
Let me know what you find out.
This was passed from Michael to John Roote.
John Roote wrote to Claudio Sunkel stating that several genes fail to
complement Df(2L)A220 and if Claudio sent the P stock they could test which
of the genes it is.
Date: Wed, 27 Mar 96 15:10:19 PST
Reply-To: np24bb@XXXXXXXXXXXXXXX (Claudio Sunkel)
From: np24bb@XXXXXXXXXXXXXXX (Claudio Sunkel)
Subject: 35B insertion
Thank you for the information. However, last week we finally sequenced part
of a cDNA clone and we found that the insertion must be very close to Su(H)
so it is very likely that it is an allele of Su(H).
Please keep the stock and if any body needs the cDNA they can have it. I
will not continue working on this mutant.
John Roote also wrote to J.M. Dura asking for more information concerning
the P-element insertion mutation at 35B.
Part of his reply:
This is a complete 10.5 kb EcoR1-Kpn1 white+ genomic DNA fragment cloned
into Carnegie 3. The mutator element was P[w-D1 X6] (19DE), a derivative
of stock 9.3 - see Delattre et al. 1995 Genetics 141:1407-1424.
Query to Kathy
Part of her reply:
The stock was from Dura, Bloomington stock number 1356, unlocalised,
inherited from HHMI, Dura called it M28/CyO.
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|Language of Publication||English|
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