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Citation
Brandes, C., Plautz, J.D., Stanewsky, R., Jamison, C.F., Straume, M., Wood, K.V., Kay, S.A., Hall, J.C. (1996). Novel features of Drosophila period transcription revealed by real-time luciferase reporting.  Neuron 16(4): 687--692.
FlyBase ID
FBrf0086927
Publication Type
Research paper
Abstract

The rapid turnover of luciferase and the sensitive, non-invasive nature of its assay make this reporter gene uniquely situated for temporal gene expression studies. To determine the in vivo regulatory pattern of the Drosophila clock gene period (per), we generated transgenic strains carrying a luciferase cDNA fused to the promoter region of the per gene. This has allowed us to monitor circadian rhythms of bioluminescence from pacemaker cells within the head for several days in individual living adults. These high time-resolution experiments permitted neuronal per transcription and opens the door to vastly simplified experiments in general chronobiology and studies of temporally regulated transcription in a wide range of experimental systems.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Neuron
    Title
    Neuron
    Publication Year
    1988-
    ISBN/ISSN
    0896-6273
    Data From Reference
    Alleles (4)
    Genes (3)
    Sequence Features (1)
    Experimental Tools (1)
    Transgenic Constructs (2)