Reference Report

Reference
Citation	Guillemin, K., Groppe, J., Ducker, K., Treisman, R., Hafen, E., Affolter, M., Krasnow, M.A. (1996). The pruned gene encodes the Drosophila serum response factor and regulates cytoplasmic outgrowth during terminal branching of the tracheal system. Development 122(5): 1353--1362. (Export to RIS)
FlyBase ID	FBrf0087274
Publication Type	Research paper
PubMed ID	8625824
PubMed Abstract	We identified a Drosophila gene, pruned, that regulates formation of the terminal branches of the tracheal (respiratory) system. These branches arise by extension of long cytoplasmic processes from terminal tracheal cells towards oxygen-starved tissues, followed by formation of a lumen within the processes. The pruned gene is expressed in terminal cells throughout the period of terminal branching. pruned encodes the Drosophila homologue of serum response factor (SRF), which functions with an ETS domain ternary complex factor as a growth-factor-activated transcription complex in mammalian cells. In pruned loss of function mutants, terminal cells fail to extend cytoplasmic projections. A constitutively activated SRF drives formation of extra projections that grow out in an unregulated fashion. An activated ternary complex factor has a similar effect. We propose that the Drosophila SRF functions like mammalian SRF in an inducible transcription complex, and that activation of this complex by signals from target tissues induces expression of genes involved in cytoplasmic outgrowth.
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (5)
	Df(2R)Px1 Df(2R)Px2 In(2LR)O In(2LR)Px⁴ T(2;3)TSTL14
Alleles (20)
	bs⁰³²⁶⁷ bs^ex3 bs^ex8 bs^ex40 bs^ex53 bs^ex84 bs^ex87 Ecol\lacZ^bs-03267 Ecol\lacZ^btl-6-81 Ecol\lacZ^Lam-02459 Ecol\lacZ^Scer\UAS.cBa Ecol\lacZ^Terminal-2 Ecol\lacZ^Terminal-3 Ecol\lacZ^Terminal-5 Ecol\lacZ^trh-1-eve-1 Hsap\ELK1^Scer\UAS.cGa Hsap\ELK1^{Scer\UAS.T:Hsim\VP16} Hsap\SRF^{Scer\UAS.T:Hsim\VP16} Scer\GAL4^C38 Scer\GAL4^tracheal
Balancers (2)
	CyO-hb-β-gal TSTL14
Constructs (4)
	P{dominant-negative.Elk-1} P{Elk-1-VP16} P{SRF-VP16} P{UAS-lacZ.B}
Genes (7)
	bs Ecol\lacZ Hsap\ELK1 Hsap\SRF Scer\GAL4 slbo T:Hsim\VP16
Insertions (6)
	P{GawB}tracheal P{lacZ}Terminal-2 P{lacZ}Terminal-3 P{lacZ}Terminal-5 P{PZ}bs⁰³²⁶⁷ P{PZ}Lam⁰²⁴⁵⁹