Reference Report

Reference
Citation	Karim, F.D., Chang, H.C., Therrien, M., Wassarman, D.A., Laverty, T., Rubin, G.M. (1996). A screen for genes that function downstream of Ras1 during Drosophila eye development. Genetics 143(1): 315--329. (Export to RIS)
FlyBase ID	FBrf0087493
Publication Type	Research paper
External Crossreferences
PubMed ID	8722784
PubMed Abstract	Cell-fate specification of the R7 photoreceptor cell is controlled by the sevenless receptor tyrosine kinase (SevRTK) and Ras1, the Drosophila homologue of mammalian H-ras, K-ras and N-ras oncogenes. An activated form of Ras1 expressed under control of the sevenless enhancer/promoter (sev-Ras1V12) induces production of supernumerary R7 photoreceptor cells, which causes the eye to become rough in appearance. To isolate mutations in genes functioning downstream of Ras1, we carried out a screen for dominant suppressors and enhancers of this rough eye phenotype. Approximately 850,000 mutagenized flies were screened, and 282 dominant suppressors and 577 dominant enhancers were isolated. Mutations in the Drosophila homologues of Raf, MEK, MAPK, type I Geranylgeranyl Transferase and Protein Phosphatase 2A were isolated, as were mutations in several novel signaling genes. Some of these mutant genes appear to be general signaling factors that function in other Ras1 pathways, while one seems to be more specific for photoreceptor development. At least two suppressors appear to function either between Ras1 and Raf or in parallel to Raf.
BIOSIS ID	99005289
Zoological Record ID
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Editors
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (4)
	Df(2R)XE-2900 Df(3L)XS705 In(2LR)O In(3LR)TM3
Alleles (65)
	14-3-3ε^S-696 14-3-3ε^S-1259 aop^E-867 aop^S2382 aop^yan-XE18 Dsor1^S-1221 Dsor1^XS520 ER2-1^E-33 ER2-1^E-203 ER2-3^XE-2213 ER2-3^XE-2542 ER2-4^XE-2171 ER2-4^XE-2866 ER2-7A^XE-2175 ER2-7A^XE-2684 ER2-7B^XE-2337 ER2-7B^XE-2457 ER3-2^XE-2551 ER3-2^XE-2795 ER3-3^XE-571 ER3-3^XE-979 ER3-4^XE-2178 ER3-4^XE-2649 ER3-5^XE-2335 ER3-5^XE-2617 hh^XE-2134 hh^XE-3161 ksr^S-627 ksr^XS-337 mam^S-639 mam^XS-2295 mts^XE-2202 mts^XE-2258 N^S-125 N^S-317 phl::tor^13D.hs.sev phl¹² phl^XS-2541 phyl²²⁴⁵ phyl^S-221 PTP-ER^XE12N Ras85D::Src64B^{V12.ΔCAAX.sev} Ras85D^N17.sev Ras85D^V12.sev Ras85D^{V12.ΔCAAX.sev} rl^S-135 rl^XS-324 SR3-2^S-432 SR3-2^XS-355 SR3-6^S-122 SR3-6^XS-328 SR3-7^S-499 SR3-7^XS-5R SR3-8^S-131 SR3-10^S-124 SR3-10^S-297 Su(Tpl)^S-192 Su(Tpl)^XS-706 Taf1^S-136 Taf1^XS-2232 Taf4^S-117 Taf4^XS-793 Taf6^XS-922 βggt-I^S-822 βggt-I^S-2554
Balancers (2)
	CyO-CR2 TM3-T2B
Constructs (5)
	P{sE-raf^tor4021} P{sevRas1.N17} P{sevRas1.V12} P{sev-Ras1^V12ΔCAAX} P{sev-Src90-Ras1^V12.ΔCAAX}
Genes (36)
	14-3-3ε aop Dsor1 ER2-1 ER2-3 ER2-4 ER2-7A ER2-7B ER3-2 ER3-3 ER3-4 ER3-5 hh ksr mam mts N phl phl::tor phyl PTP-ER Ras85D Ras85D::Src64B rl SR3-2 SR3-6 SR3-7 SR3-8 SR3-10 Src64B Su(Tpl) Taf1 Taf4 Taf6 tor βggt-I
Insertions (2)
	P{sevRas1.V12}FK1 P{sevRas1.V12}FK2