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Reference
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| Citation |
Hopmann, R., Cooper, J.A., Miller, K.G. (1996). Actin organization, bristle morphology, and viability are affected by actin capping protein mutations in Drosophila. J. Cell Biol. 133(6): 1293--1305. |
| FlyBase ID |
FBrf0088213 |
| Type of publication |
Research paper |
| Offprint Available |
No |
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External Crossreferences
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| PubMed ID |
8682865 |
| PubMed Abstract |
Regulation of actin filament length and orientation is important in many actin-based cellular processes. This regulation is
postulated to occur through the action of actin-binding proteins. Many actin-binding proteins that modify actin in vitro have
been identified, but in many cases, it is not known if this activity is physiologically relevant. Capping protein (CP) is
an actin-binding protein that has been demonstrated to control filament length in vitro by binding to the barbed ends and
preventing the addition or loss of actin monomers. To examine the in vivo role of CP, we have performed a molecular and genetic
characterization of the beta subunit of capping protein from Drosophila melanogaster. We have identified mutations in the
Drosophila beta subunit-these are the first CP mutations in a multicellular organism, and unlike CP mutations in yeast, they
are lethal, causing death during the early larval stage. Adult files that are heterozygous for a pair of weak alleles have
a defect in bristle morphology that is correlated to disorganized actin bundles in developing bristles. Our data demonstrate
that CP has an essential function during development, and further suggest that CP is required to regulate actin assembly during
the development of specialized structures that depend on actin for their morphology.
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| Biosis |
99083974 |
| Zoological record |
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Associated Information
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| Text of personal communication |
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Related Publications
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Also Published As
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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| Place of publication |
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Published In
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| Abbreviation |
J. Cell Biol. |
| Title |
Journal of Cell Biology |
| Authors |
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| Volume range |
1- |
| Year range |
1966- |
| Page range |
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| Publisher |
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| Place of publication |
New York |
| Language of publication |
English |
| ISBN/ISSN |
0021-9525 |
| CODEN |
JCLBA3 |
Data from Reference
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Aberrations (2)
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Alleles (4)
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Constructs (1)
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Genes (2)
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