Reference Report

Reference
Citation	Kim, J., Shen, B., Rosen, C., Dorsett, D. (1996). The DNA-binding and enhancer-blocking domains of the Drosophila suppressor of Hairy-wing protein. Mol. Cell. Biol. 16(7): 3381--3392. (Export to RIS)
FlyBase ID	FBrf0088296
Publication Type	Research paper
PubMed ID	8668153
PubMed Abstract	Mutations in the suppressor of Hairy-wing [su(Hw)] gene of Drosophila melanogaster can cause female sterility and suppress mutations that are insertions of the gypsy retrotransposon. Gypsy binds the protein (SUHW) encoded by su(Hw), and SUHW prevents enhancers promoter-distal to gypsy from activating gene transcription. SUHW contains 12 zinc fingers flanked by acidic N- and C-terminal domains. We examined the roles of each of the 12 zinc fingers in binding gypsy DNA and classified them into four groups: essential (fingers 6 through 10); beneficial but nonessential (fingers 1, 2, 3, and 11); unimportant (fingers 5 and 12); and inhibitory (finger 4). Because finger 10 is not required for female fertility but is essential for binding gypsy, these results imply that the SUHW-binding sites required for oogenesis differ in sequence from the gypsy-binding sites. We also examined the functions of the N- and C-terminal domains of SUHW by determining the ability of various deletion mutants to support female fertility and to alter expression of gypsy insertion alleles of the yellow, cut, forked, and Ultrabithorax genes. No individual segment of the N- and C-terminal domains of SUHW is absolutely required to alter expression of gypsy insertion alleles. However, the most important domain lies between residues 737 and 880 in the C-terminal domain. This region also contains the residues required for female fertility, and the fertility domain may be congruent with the enhancer-blocking domain. These results imply that SUHW blocks different enhancers and supports oogenesis by the same or closely related molecular mechanisms.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Cell. Biol.
Title	Molecular and Cellular Biology
Publication Year	1981-
ISBN/ISSN	0270-7306
Data from Reference
Aberrations (1)
	Df(3R)su(Hw)V
Alleles (21)
	ct⁶ f¹ RpII15^tKa RpII15^V su(Hw)⁷ su(Hw)^e2 su(Hw)^E8 su(Hw)^V su(Hw)^wt su(Hw)^{Δ2-203.673-943} su(Hw)^Δ2-203 su(Hw)^Δ656-705 su(Hw)^Δ673-943 su(Hw)^Δ706-736 su(Hw)^Δ738-780 su(Hw)^Δ782-944 su(Hw)^Δ853-880 su(Hw)^Δ881-944 Ubx^bx-34e w^+mC y²
Constructs (10)
	P{su(Hw)wt} P{Δ2-203;673-943} P{Δ2-203} P{Δ656-705} P{Δ673-943} P{Δ706-736} P{Δ738-780} P{Δ782-944} P{Δ853-880} P{Δ881-944}
Genes (8)
	ct f gypsy\su(Hw)BR RpII15 su(Hw) Ubx w y
Insertions (4)
	gypsy{}ct⁶ gypsy{}f¹ gypsy{}Ubx^bx-34e gypsy{}y²