|Citation||Shearn, A. (1996.6.25). y+ CyO chromosome. (Export to RIS)|
|Publication Type||Personal communication to FlyBase|
|Text of Personal Communication||
From rd120@XXXXXXXXXXXXXXX Tue Jun 25 14:29:55 1996
From: Rachel Drysdale (Genetics) <rd120@XXXXXXXXXXXXXXX>
Date: Tue, 25 Jun 96 14:29:49 BST
Subject: helping FlyBase
I have a question about a marked balancer that you published in 1993. It
is the y+ CyO chromosome published in Timmons et al., 1993, Dev. Biol.
It was said to have been constructed by Elizabeth Woodhouse (Materials and
Methods, bottom of page 365).
Has this element been previously published? If so, can you tell me which
y+ construct was used? We have records for many y+ constructs in
FlyBase so its possible that we already have an entry for it, and I would
like to link this balancer to the correct construct, if at all possible.
If it is one that Elizabeth made herself, if you could let me know a couple
of details, such as size of yellow genomic DNA fragment, and transformation
vector, then that would be wonderful.
many thanks for your help,
with best wishes,
From bio_cals@XXXXXXXXXXXXXXX Tue Jun 25 15:13:35 1996
Date: Tue, 25 Jun 1996 10:02:04 -0400 (EDT)
Date-Warning: Date header was inserted by jhmail.hcf.jhu.edu
From: bio_cals@XXXXXXXXXXXXXXX (Allen Shearn)
Subject: Re: helping FlyBase
To: Rachel Drysdale <rd120@XXXXXXXXXXXXXXX> (Genetics)
Content-Type: text/plain; charset='us-ascii'
The y+ construct in a P-element vector was made by Pam Geyer at The
University of Iowa. The insert we started with was on an otherwise unmarked
2nd chromosome. I am afraid that I do not know the details of the
construct. We have subsequently transposed the same insert onto a TM3
chromosome that is marked with Ser and e but not Sb. The y+ has been
completely stable on both balancers.
Department of Biology Johns Hopkins University Baltimore, MD 21218
PHONE 410-516-7285 FAX 410-516-5213
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|Language of Publication||English|
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