Reference Report

Reference
Citation	Martin-Bermudo, M.D., Brown, N.H. (1996). Intracellular signals direct integrin localization to sites of function in embryonic muscles. J. Cell Biol. 134(1): 217--226. (Export to RIS)
FlyBase ID	FBrf0089738
Publication Type	Research paper
PubMed ID	8698816
PubMed Abstract	In the Drosophila embryo, the alphaPS2betaPS integrin heterodimer is localized tightly at the termini of the multinucleate muscles where they attach to the alphaPS1betaPS-containing epidermal tendon cells. Here we examine the basis for alphaPS2betaPS integrin subcellular localization. We show that the betaPS cytoplasmic tail is sufficient to direct the localization of a heterologous transmembrane protein, CD2, to the muscle termini in vivo. This localization does not occur via an association with structures set up by the endogenous betaPS integrins, since it can occur even in the absence of the betaPS protein. Furthermore, the subcellular localization of the alphaPS2betaPS integrin is not dependent on any other interactions between the muscles and the tendon cells. In embryos that lack the segmental tendon cells, due to a mutation removing the related segment polarity genes engrailed and invected, alphaPS2betaPS is still localized to the muscle termini even though the ventral longitudinal muscles are not attached to the epidermis, but instead are attached end to end. Thus the alphaPS2betaPS integrin can be localized by an intracellular mechanism within the muscles. Our results challenge the view that the transmission of signals from the extracellular environment via integrins is required for the organization of the cytoskeleton and the resultant cellular polarity.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (1)
	Df(2R)en^E
Alleles (13)
	Ecol\lacZ^en-Xho25 Ecol\lacZ^sr-B14.0 if^B4 mys¹¹ mys^{CCβ.Scer\UAS.T:Rnor\CD2} mys^{Cββ.Scer\UAS.T:Rnor\CD2} mys^din.Scer\UAS mys^din Rnor\CD2^{Scer\UAS.cDBa} Scer\GAL4^how-24B Scer\GAL4^twi.PG sr¹⁶ sr^B14.0
Constructs (4)
	P{CCβ} P{Cββ} P{GAL4-twi.G} P{UAS-mys.din}
Genes (9)
	Ecol\lacZ en if inv mys Rnor\CD2 Scer\GAL4 sr T:Rnor\CD2
Insertions (3)
	P{en-lacZ(Xho)}en^Xho25 P{GawB}how^24B P{lwB}sr^B14.0