Reference Report

Reference
Citation	Sun, X., Artavanis-Tsakonas, S. (1996). The intracellular deletions of Delta and Serrate define dominant negative forms of the Drosophila Notch ligands. Development 122(8): 2465--2474. (Export to RIS)
FlyBase ID	FBrf0089839
Publication Type	Research paper
PubMed ID	8756291
PubMed Abstract	We examined the function of the intracellular domains of the two known Drosophila Notch ligands, Delta and Serrate, by expressing wild-type and mutant forms in the developing Drosophila eye under the sevenless promoter. The expression of intracellularly truncated forms of either Delta (sev-DlTM) or Serrate (sev-SerTM) leads to extra photoreceptor phenotypes, similar to the eye phenotypes associated with loss-of-function mutations of either Notch or Delta. Consistent with the notion that the truncated ligands reduce. Notch signalling activity, the eye phenotypes of sev-DlTM and sev-SerTM are enhanced by loss-of-function mutations in the Notch pathway elements, Notch, Delta, mastermind, deltex and groucho, but are suppressed by a duplication of Delta or mutations in Hairless, a negative regulator of the pathway. These observations were extended to the molecular level by demonstrating that the expression of Enhancer of split m delta, a target of Notch signalling, is down-regulated by the truncated ligands highly expressed in neighbouring cells. We conclude that the truncated ligands act as antagonists of Notch signalling.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (4)
	Df(1)N-54l9 Dp(3;3)bxd¹¹⁰ In(3R)Ser^rv2-11 Tp(3;3)bxd¹¹⁰
Alleles (13)
	Dl^{ICS.MtnA.T:Hsap\MYC} Dl^{ICS.sev.T:Hsap\MYC} Dl^MtnA.PF Dl^sev.PS Dl^TM.sev Dl^{TMIC.sev.T:Hsap\MYC} H¹ N^nucl.sev Ser^MtnA.PR Ser^rev2-11 Ser^sev.T:VSV\G Ser^{TM.sev.T:VSV\G} w^+mC
Constructs (8)
	P{sev-Dl} P{sev-DlICS} P{sev-DlTM} P{sev-DlTMIC} P{sev-Notch^nucl} P{sev-Ser} P{sev-SerTM} pP{sevE}
Genes (14)
	B Dl dx E(spl)mγ-HLH elav gro H mam N ro Ser T:Hsap\MYC T:VSV\G w