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Citation
Tear, G., Harris, R., Sutaria, S., Kilomanski, K., Goodman, C.S., Seeger, M.A. (1996). commissureless controls growth cone guidance across the CNS midline in Drosophila and encodes a novel membrane protein.  Neuron 16(3): 501--514.
FlyBase ID
FBrf0089842
Publication Type
Research paper
Abstract

The commissureless (comm) gene was identified previously in a large-scale screen for mutations that disrupt CNS axon pathways in Drosophila. The comm gene has a unique mutant phenotype: the complete absence of most axon commissures, while midline cells and other aspects of CNS fate and patterning are left unchanged. Here, we report on the molecular cloning, characterization, and expression of the comm gene. comm encodes a novel protein of 370 amino acids that lacks a signal sequence, has a transmembrane domain, and biochemically copurifies with membranes. COMM mRNA and COMM protein are dynamically expressed during embryogenesis, including by CNS midline glia during the formation of the axon commissures. Anti-COMM antibodies reveal strong staining of organelles likely to include the Golgi complex and endosomes and weaker staining of the cell surface. As commissural growth cones contact and traverse the CNS midline, COMM protein is apparently transferred from midline glia to commissural axons.

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Headlines.
Anonymous, 1996, Trends Cell Biol. 6(6): 216 [FBrf0099707]

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Neuron
    Title
    Neuron
    Publication Year
    1988-
    ISBN/ISSN
    0896-6273
    Data From Reference
    Aberrations (4)
    Alleles (8)
    Genes (2)
    Cell Lines (1)