Reference Report

Reference
Citation	Fujioka, M., Miskiewicz, P., Raj, L., Gulledge, A.A., Wier, M., Goto, T. (1996). Drosophila Paired regulates late even-skipped expression through a composite binding site for the paired domain and the homeodomain. Development 122(9): 2697--2707. (Export to RIS)
FlyBase ID	FBrf0090021
Publication Type	Research paper
PubMed ID	8787744
PubMed Abstract	The even-skipped (eve) pair-rule gene plays a key role in the establishment of the anterior-posterior segmental pattern of the Drosophila embryo. The continuously changing pattern of eve expression can be resolved into two phases. Early expression consists of seven broad stripes in the blastoderm embryo, while late expression, which occurs after cellularization, consists of narrow stripes with sharp anterior borders that coincide with the odd-numbered parasegment boundaries. Previous studies have shown that these two phases are controlled by separate classes of cis elements in the eve promoter. Early stripes are expressed by multiple stripe-specific elements under the control of maternal-effect genes and gap genes, while late stripes are expressed by a single regulatory element, the 'late element', under the control of pair-rule genes including eve itself. We report here that paired (prd), a pair-rule gene which had been considered to be below eve in the regulatory hierarchy of pair-rule genes, in fact plays a critical role in the regulation of late eve expression. Transgenic analysis shows that this regulation is largely mediated by an evolutionarily conserved sequence within the late element termed PTE (Paired Target Element). In vitro analysis shows that the Prd protein binds strongly to this sequence. Interestingly, PTE contains juxtaposed binding sites for the two DNA-binding domains of the Prd protein, the paired domain and the homeodomain. Mutagenesis of either binding site leads to significant reduction in the activity of the late element, indicating that both DNA-binding domains in the Paired protein are required for regulation.
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (1)
	In(2LR)O
Alleles (21)
	Ecol\lacZ^Dhyd\eve.L Ecol\lacZ^{Dhyd\eve.LmLd} Ecol\lacZ^eve.32Bsu Ecol\lacZ^eve.32ΔBsu Ecol\lacZ^eve.41ΔSac Ecol\lacZ^eve.C3DZ Ecol\lacZ^eve.Lm Ecol\lacZ^eve.LmLd Ecol\lacZ^eve.PstBsu Ecol\lacZ^eve.PstΔBsu Ecol\lacZ^eve.PstΔSac Ecol\lacZ^eve.RVBsu Ecol\lacZ^eve.RVSac Ecol\lacZ^eve.RVΔ41S Ecol\lacZ^eve.RVΔPS Ecol\lacZ^eve.RVΔSac Ecol\lacZ^eve.RΔMAS Ecol\lacZ^eve.ΔSAMS prd⁴ prd^hs.PM w^+mC
Balancers (1)
	CyO-Thb8
Constructs (19)
	P{32Bsu} P{32ΔBsu} P{41ΔSac} P{C3DZ} P{Dhyd-L} P{Dhyd-LmLd} P{hs-prd.M} P{Lm} P{LmLd} P{PstBsu} P{PstΔBsu} P{PstΔSac} P{RVBsu} P{RVSac} P{RVΔ41S} P{RVΔPS} P{RVΔSac} P{RΔMAS} P{ΔSAMS}
Genes (5)
	Dhyd\eve Ecol\lacZ eve prd w
Sequence features (2)
	eve_32Bsu eve_Lm