Reference Report

Reference
Citation	Dahanukar, A., Wharton, R.P. (1996). The Nanos gradient in Drosophila embryos is generated by translational regulation. Genes Dev. 10(20): 2610--2620. (Export to RIS)
FlyBase ID	FBrf0090514
Publication Type	Research paper
PubMed ID	8895662
PubMed Abstract	Abdominal segmentation in the Drosophila embryo is governed by a gradient of Nanos (Nos) emanating from the posterior pole. This gradient is derived from translation of the nos mRNA that is localized in the pole plasm; in contrast, unlocalized nos mRNA is translationally repressed. Here we define the essential signals in the 3' untranslated region (UTR) of nos mRNA. Deletion of a 184-nucleotide translational control element (TCE) from the 3' UTR leads to the derepression of nos mRNA in the bulk cytoplasm and the development of lethal anterior defects. Furthermore, a minimal mRNA containing essentially only the TCE in its 3' UTR rescues nos- embryos to adulthood. The TCE is also sufficient to confer on maternal torso mRNA all three aspects of nos mRNA regulation: translational repression in the bulk cytoplasm, localization to the pole plasm, and translational activation at the posterior pole. These three phenomena are coupled intimately, as mutations in a pair of CUGGC pentamers within the TCE simultaneously abrogate all three regulatory events. This coupling suggests a model in which the polarized distribution of nos protein is generated primarily by translational control and that nos mRNA localization is a byproduct of this regulation, at least in part.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Alleles (30)
	bcd⁶ nos^+t5.7 nos^bcd.3UTR.D nos^BN nos^TCE1 nos^TCE2 nos^TCE3 nos^TCE4 nos^TCE5 nos^TCE6 nos^TCE7 nos^TCE8 nos^TCE9 nos^TCE.bcdUTR nos^ΔBE nos^ΔBX nos^ΔBX-tetra2 nos^ΔEE nos^ΔEX nos^ΔTCE nos^ΔTCE-A nos^ΔTCE-B nos^ΔX.αTub84B osk⁶ tor^+t11.5 tor^rv66 tor^TCE.Asp718 tor^TCE.SpeI vas¹ w^+mC
Constructs (24)
	P{nos(ΔBE)} P{nos(ΔBX,tetra²)} P{nos(ΔBX)} P{nos(ΔEE)} P{nos(ΔEX)} P{nos(ΔTCE)} P{nos(ΔTCE-A)} P{nos(ΔTCE-B)} P{nos(ΔX+αTub84B)} P{nos⁺} P{nos-bcd} P{nos-TCE-bcd} P{TCE1} P{TCE2} P{TCE3} P{TCE4} P{TCE5} P{TCE6} P{TCE7} P{TCE8} P{TCE9} P{tor(TCE)Asp718} P{tor(TCE)SpeI} P{tor⁺.S}
Genes (7)
	bcd hb nos osk tor vas w