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Reference
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| Citation |
Eaton, S., Wepf, R., Simons, K. (1996). Roles for Rac1 and Cdc42 in planar polarization and hair outgrowth in the wing of Drosophila. J. Cell Biol. 135(5): 1277--1289. |
| FlyBase ID |
FBrf0090541 |
| Type of publication |
Research paper |
| Offprint Available |
No |
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External Crossreferences
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| PubMed ID |
8947551 |
| PubMed Abstract |
The wing of Drosophila melanogaster is covered by an array of distally pointing hairs. A hair begins as a single membrane
outgrowth from each wing epithelial cell, and its distal orientation is determined by the restriction of outgrowth to a single
distal site on the cell circumference (Wong, L., and P. Adler. 1993. J. Cell Biol. 123:209-211.). We have examined the roles
of Cdc42 and Rac1 in the formation of wing hairs. We find that Cdc42 is required for localized actin polymerization in the
extending hair. Interfering with Cdc42 activity by expression of a dominant negative protein abolishes both localized actin
polymerization and hair outgrowth. In contrast, Rac1 is important for restricting the site at which hairs grow out. Cells
expressing the dominant negative Rac1N17 fail to restrict outgrowth to a single site and give rise to multiple wing hairs.
This polarity defect is associated with disturbances in the organization of junctional actin and also with disruption of an
intricate microtubule network that is intimately associated with the junctional region. We also find that apical junctions
and microtubules are involved in structural aspects of hair outgrowth. During hair formation, the apical microtubules that
point distally elongate and fill the emerging wing hair. As the hair elongates, junctional proteins are reorganized on the
proximal and distal edges of each cell.
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| Biosis |
99336151 |
| Zoological record |
13605001342 |
Associated Information
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| Text of personal communication |
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Also Published As
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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| Place of publication |
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Published In
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| Abbreviation |
J. Cell Biol. |
| Title |
Journal of Cell Biology |
| Authors |
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| Volume range |
1- |
| Year range |
1966- |
| Page range |
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| Publisher |
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| Place of publication |
New York |
| Language of publication |
English |
| ISBN/ISSN |
0021-9525 |
| CODEN |
JCLBA3 |
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Data from Reference
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| Alleles (3)
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| Constructs (2)
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| Genes (12)
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| Insertions (1)
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