Reference Report

Reference
Citation	Rorth, P. (1996). A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422. (Export to RIS)
FlyBase ID	FBrf0090768
Publication Type	Research paper
External Crossreferences
PubMed ID	8901596
PubMed Abstract	Genetic screens in Drosophila have lead to the discovery of many genes important for patterning and signal transduction in diverse organisms. Traditionally, the phenotypic effects of loss-of-function mutations are analyzed. As an alternative way to link genes and function, I have developed a versatile misexpression screen in Drosophila, the first such screen in higher eukaryotes. The screen identifies genes that, when over- or misexpressed in a pattern of interest, give a specific phenotype or modulate an existing mutant phenotype. It is based on Gal4 transactivation of a mobile enhancer and promoter that "targets" random endogenous genes for expression. The modular design of the screen allows directed expression in any temporal or spatial pattern. When activated in the developing eye, 4% of target inserts gave dominant phenotypes. One insertion was in the gene encoding Ras GTPase-activating protein; its overexpression phenotype was strongly enhanced by a mutation in Ras1. Thus, biologically relevant phenotypes and genetic interactions are identified using this method. The screen is a powerful new tool for developmental genetics; similar approaches can also be developed for other organisms.
BIOSIS ID	99261179
Zoological Record ID
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FB2012_01	Insertions P{EP}puc[EP3194] P{EP}GE10558 P{EP}mgl[GE3293] P{EP}l(3)S055409[S055409]
FB2011_10
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Text of Personal Communication
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Related Publications
Review	Monitor. Anonymous, 1997, Trends Genet. 13(2): 53 [FBrf0092775]
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
ISBN
Parent Publication
Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Editors
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (21)
	anon-42Ea^EPS100 anon-50Aa^EPS64 anon-92Da^EPS111 Ecol\lacZ^Hsp70Bb.HZ50 Ecol\lacZ^Scer\UAS.cRa EP115^EP115 Gap1^EP45 gypsy\su(Hw)BR³⁷⁰ peb^EP55 Ras85D⁰⁵⁷⁰³ ry^+t7.2 Scer\GAL4^32B Scer\GAL4^69B Scer\GAL4^h-1J3 Scer\GAL4^how-24B Scer\GAL4^hs.2sev Scer\GAL4^T80 Scer\UAS^14x.hs Scer\UAS^14x TrlBR^cRa w^+mC
Constructs (18)
	14xUAS_G 001268 001269 001271 001272 Hsp70Bb.P(0.078) P{EP} P{GAL4-Hsp70.sev} P{Mae-UAS.E} P{Mae-UAS.E^S} P{Mae-UAS.EP^S} P{UAS-lacZ.HZ50} pHSS7(N,R1)b pP{EP} pP{Mae-UAS.E} pP{Mae-UAS.E^S} pP{Mae-UAS.EP^S} pP{UAS-lacZ.HZ50}
Genes (14)
	anon-42Ea anon-50Aa anon-92Da Ecol\lacZ EP115 Gap1 gypsy\su(Hw)BR peb Ras85D ry Scer\GAL4 Scer\UAS TrlBR w
Insertions (12)
	P{EP}EP115^EP115 P{EP}Gap1^EP45 P{EP}peb^EP55 P{GawB}32B P{GawB}69B P{GawB}h^1J3 P{GawB}how^24B P{GawB}T80 P{Mae-UAS.EP^S}anon-42Ea^EPS100 P{Mae-UAS.EP^S}anon-50Aa^EPS64 P{Mae-UAS.EP^S}anon-92Da^EPS111 P{PZ}Ras85D⁰⁵⁷⁰³